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IG.
3. A, Northern blots showing hybridization to RNA isolated
from various developmental stages of X. laeuis. Each lane contains
poly(A’) RNA from five embryo equivalents. The top panel shows
hybridization with “LP-labeled antisense TGF-05 RNA, the bottom
panel with “‘P-labeled antisense Vgl RNA. Size markers show that
the TGF-85 transcript is about 3.0 kb; the Vgl transcript is about 2.8
kb. B, Northern blot analysis showing hybridization of “‘P-labeled
TGF-45 cDNA to RNAs derived from various tissues of adult X.
laeuis and from XTC cells. Each lane contains 15 pg of total RNA,
except lane 6, which has 5 pg. RNAs were resolved in 1.2% agarose
gels containing 2.2 M formaldehyde in 0.04 M MOPS buffer (pH 7.0).
Respective tissues are labeled above each lane. |
