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Figure 6. . GCAP1 immunolabeling in dark-adapted Xenopus retina. (A) In larval retinas (stage 48), anti-GCAP1 labeling was found mainly in cone photoreceptors with very weak labeling of rod outer segments. (B) In froglet retinas, anti-GCAP1 labeling was found in both cone and rod photoreceptors, although labeling was significantly stronger in cones than in rods. Note that GCAP1 staining in cones also increased as the retinas matured. In (C) control tadpole and (D) froglet retinas probed with secondary antibody, fluorescence levels can be hardly detected. All confocal images were processed identically. |
