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Fig. 2. Distribution of CARTp-immunoreactive cells and fibers in
the telencephalon and the preoptic area. A: Immunostaining in the
main olfactory bulb. Only a few cells are stained in the internal
granular layer (igl). Sagittal section. B: Immunoreactive perikarya in
the medial pallium (mp) and the septum (s). Sagittal section. C: Immunoreactive
neurons and fiber terminals in the nucleus accumbens
(Acc) and the striatum (Str). D: Three groups of immunoreactive
neurons in the preoptic area (POa). Sagittal section. E: Intensely
stained neurons in the magnocellular preoptic nucleus (Mg). Note
caudal extent of the nucleus over the optic chiasm (oc). Sagittal
section. gl, glomerular layer; lv, lateral ventricle; ml, mitral cell layer;
olfn, olfactory nerve. Scale bar 100 m in A; 200 m in b–E. |
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Fig. 3. CARTp-immunoreactive perikarya in the diencephalon and
the mesencephalon. A: Immunoreactivity in the rostral diencephalon.
Note stained cells in the nucleus of Bellonci (B; arrows). B: Immunoreactive
cells and fibers in the infundibulum (inf) and the dorsal
hypothalamic nucleus (dHy). C: Stained cells of the magnocellular
preoptic nucleus (Mg) in the wall of the infundibulum (inf). Note that
their long dendrites are oriented laterally, toward the surface of the
brain. D: Immunoreactive cells in the hypothalamus and the Edinger-
Westphal (EW) nucleus and intensely stained axon terminals in the
neural lobe of the pituitary gland (pn). Sagittal section. Rostral is to
the left. E: Immunostained infundibular neurons with long dendrites
at the level of plane E in Figure 1. Note stained perikarya in the
ventromedial (VM) and the posterior entopeduncular nuclei (epp).
F: Immunostained fibers in the pars nervosa (pn) of the pituitary
gland and median eminence of the hypothalamus. The intermediate
pituitary lobe (pi) is immunonegative, whereas some cells in the distal
lobe (pd) reveal weak immunoreactivity. G: Detail of the median
eminence, with small and less numerous stained dots in the internal
zone (iz) and large stained dots and short varicose fibers in the
external zone (ez). Hd, dorsal habenula; Hv, ventral habenula. 3rd,
third ventricle. Scale bar 200 m in A,B; 100 m in C,E; 300 m in
D; 150 m in F; 15 m in G. |
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Fig. 4. CARTp-immunoreactivity in the mesencephalon and
rhombencephalon. A: Pretectal region. Note heavy staining of neurons
in the central thalamic nucleus (C). B: Optic tectum. Note stained
cells in layer 9 and intense fiber staining in the periventricular layers
(pvl). C: Immunostained fibers and cells in the optic tectum (tect) and
the magnocellular nucleus (mt) of the torus semicircularis. D: Sagittal
section of the mesencephalon and the rhombencephalon. Note intense
fiber staining in the tectum and fasciculus retroflexus (arrow), and
heavily stained neurons in the central thalamic nucleus (C). E: Intense
fiber staining in the isthmic region. Note that the isthmic (Is)
and the interpeduncular (Ip) nuclei are free of immunostaining.
F: Stained cells and fibers in the superior raphe nucleus (r). aq,
cerebral aqueduct; cer, cerebellum; cg, central gray; Lpv, lateral thalamic
nucleus, posteroventral division; P, posterior thalamic nucleus;
Pn, pretectal neuropil; Pv, posteroventral tegmental nucleus; 4th,
fourth ventricle. For other abbreviations, see list. Scale bar 300 m
in A; 100 m in B,F; 200 m in C,E; 500 m in D. |
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Fig. 5. CARTp-immunoreactivity in the rhombencephalon and the
spinal cord. A: Neurons in the nucleus of the solitary tract (nst,
arrow). B: Intense staining of fiber terminals in the ambiguous nucleus
(IX–Xm, arrow). Note stained cells dorsomedially. C: Neurons in
the gracile nucleus (Ng) of the dorsal column nuclei. D: Fiber terminals
and neurons in the intermediate zone of the spinal cord. Horizontal
section. E: Stained cells (arrows) medial and dorsal to the
lateral motoneurons in the spinal cord in a lumbar segment. Note
fiber staining in the dorsal horn (dh), and among unstained motoneurons
(vh). The midline is to the left. af, anterior funiculus; cc, central
canal; cg, central gray; lf, lateral funiculus; pf, posterior funiculus; sol,
nucleus of the solitary tract; 4th, fourth ventricle. Scale bar 100 m
in A–D; 200 m in E. |
