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Fig. 2. Expression patterns of xier2. (A) RT-PCR analysis of Xenopus ier2a and b genes from early cleavage to st 40. Odc was used as control. (B-I) Whole mount in situ hybridization of xier2b, using antisense strand probe; (J-L) sense strand used as control. (B,C) Maternal expression is seen in whole mount and transverse section of two-cell stage embryo (C). (D,E) Restricted expression of xier2b in the marginal zone and involuting axial mesoderm during gastrulation at stage 11. (F,G) Hindbrain and somite expression of xier2 at st 13 (F) and st 25 (G). (H,I) Expression of xier2b in branchial arches, notochord, and somites at stage 30; (I) is a magnified view of the head region. ba, branchial arches; h, hindbrain; no, notochord, so, somites. (J-L) Embryos at st 11 (J,K) and st 29/30 (L) hybridized with sense strand as controls. |
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ier2 (immediate early response 2) gene expression in Xenopus laevis embryo via in situ hybridization, NF stage 25, lateral view, anterior left. |
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ier2 (immediate early response 2) gene expression in Xenopus laevis embryo via in situ hybridization, NF stage 29 to stage 30, lateral view, |
