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Figure 1. Whole-mounts of stage 53 and stage 57 Xenopus hindlimbs from 0 hours to 5 days post-amputation (PA) stained by enzyme histochemistry for myeloperoxidase (MPO), a marker for neutrophils and macrophages. Only background staining is visible at the time of amputation (0 hPA), but by 6 hPA in limbs of both stages the number of brown MPO+ cells increase greatly near the site of amputation. MPO staining diminished slowly through 5 dPA in limbs of both stages. The larger, very dark structures are melanocytes. |