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Figure S1. Whole mount in situ hybridization (WISH) of select vegetal and animal pole transcripts. Expression of a subset of RNAs enriched in the vegetal (A-B) and animal (C) poles were analyzed during oogenesis and development by WISH. Probes, developmental stages, and developmental structures are indicated: germplasm/PGCs (purple), pronephros (pink), ventral blood islands (lime green), eye (black), lens (white), otic vesicle (gray), cranial ganglia (yellow), brachial arches (green), nasal placodes (teal), inter-segmental region (brown), notochord (orange), brain and neural tube (blue). Transcripts detected in primordial germ cells
at the tailbud stage are magnified and embedded in their respective images. Black bars represent 100um in st. I oocyte panels, and 200um in all other panels. Please refer to Fig. 3 for
use of xpat probe as a positive control for germ plasm localization. |
