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Gene/Clone	Species	Stage	Anatomy Item	Experimenter
tph1	xenopus		foregut [+] acinar cell,alpha cell,antrum,beta cell,cardia,delta cell,distal stomach,epsilon cell,exocrine pancreas,fundus,gamma cell,islets of Langerhans,proximal stomach,pulmonary epithelium,pyloric sphincter,pylorus,stomach body,buccopharyngeal membrane,first pancreatic bud,islets of Langerhans,lung,lung bud,lung primordium,oral evagination,pancreas,pharyngeal cavity,pyloric sphincter,second pancreatic bud,stomach,stomach epithelium,taste bud,thyroid,thyroid primordium,ventral pancreatic bud,visceral pouch

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Experiment details for tph1

Walentek P et al. (2015) Assay

ATP4a is required for development and function of the Xenopus mucociliary epidermis - a potential model to study proton pump inhibitor-associated pneumonia.

Gene	Clone	Species	Stages	Anatomy
tph1.L		laevis	NF stage 32	foregut

Fig. 5. Serotonin secretion and tph1 expression in the embryonic epidermis are regulated by ATP4a-mediated Wnt/β-catenin signaling. (A–D) Immunofluorescent analysis and quantification (E) of serotonin (5-HT; red) deposition in small secretory cells (SSCs) of the skin at stage 32. Actin (phalloidin-Alexa488) staining in green. (A) Uninjected (uninj.) control. (B) Loss of serotonin staining in atp4a morphants. (C,D) Rescue of serotonin staining upon co-injection of atp4a (C) or β-catenin (β-cat.; D) DNA constructs. (A‵–D‵) Serotonin channel. (A‶–D‶) Higher magnification of (A–D). (E) Quantification of results. (F–H) WMISH for tph1. (F, F‵) Uninjected control. (G, G‵) Loss of tph1 expression in atp4a morphants was rescued by co-injection of atp4a DNA (H) (n, number of embryos; st., stage).

Gene	Clone	Species	Stages	Anatomy
tph1.L		laevis	NF stage 32	foregut

tph1 (tryptophan hydroxylase 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 32, lateral view, anterior right, dorsal up.