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Fig. 7.
Xenopus Kuzbanian is expressed in the developing diencephalic brain neuroepithelium.A, Western blot of adult rat cerebellar (left) and stage 42 Xenopus head (right) tissue using a rabbit antibody developed against a human ADAM10 peptide. Molecular masses are indicated based on standards run at the same time as the sample tissue. A prominent protein band with an approximate molecular mass of 82 kDa was observed in rats, and a similarly sized band of ∼75 kDa was observed inXenopus. B, C, Immunolabeling of stage 35/36 Xenopus transverse sections with the ADAM10 antibody shows labeling of cells in the diencephalon (B) and in the RGC layer (C). Note that there is no immunoreactivity in the neuropil or the optic nerve. Np, Neuropil;Di, diencephalon; PE, pigment epithelium;RGCL, RGC layer; L, lens;D, dorsal; V, ventral. Scale bars, 50 μm. |
