|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
appl2
|
|
laevis
|
NF stage 7
to
NF stage 11
|
animal hemisphere
,
blastocoel roof
|
appl2
|
|
laevis
|
NF stage 17
to
NF stage 21
|
neural crest
,
cement gland primordium
,
neural plate
,
anterior neural fold
,
trunk neural crest
,
[+]
|
appl2
|
|
laevis
|
NF stage 32
|
brain
,
forebrain
,
midbrain
,
hindbrain
,
cement gland
,
[+]
|
appl2
|
|
laevis
|
NF stage 35 and 36
|
brain
,
forebrain
,
midbrain
,
hindbrain
,
cement gland
,
[+]
|
|
|
Supp. Fig. S2. Spatial and temporal expression of appl2 in Xenopus embryos. A–I: Spatial expression of appl2 in Xenopus embryos revealed by whole mount in situ hybridization. A: Lateral view of a blastula embryo with signals mainly detected in the animal hemisphere. B: Bisected half of a gastrula embryo showing weak and ubiquitous expression. C: Anterodorsal view. D: Anterior view. E: Lateral view. F: Dorsal view. G–I: Dissected guts probed with appl2. In comparison to those observed for appl1 (Fig. 1G–I) and akt2 (Fig. 5A), only faint signals in the pancreas and SD were detected after a much longer period of color reaction incubation during the last step of whole mount in situ hybridization. J: RT-PCR analysis reveals the temporal expression of appl2. UE, unfertilized egg; N, negative control. ODC was used as the RNA loading control. |