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Fig. 3. The localization of arrestin in wild-type tadpoles detected immunocytochemically is consistent with that of Ar-GFP in transgenic tadpoles. Sections
from wild-type Xenopus tadpole retinas were labelled using an anti-arrestin monoclonal antibody xAr1-6 (red channel). Immunocytochemically detected
arrestin is localized in the IS and axonemes of dark-adapted retinas (DA (ON)), in a banded, proximal-to-distal gradient in the OS during early light adaptation
LA (450), and in the IS and axonemes after extended light adaptation LA (2400) at 860 lux. Photoreceptors from retinal sections stained with secondary antibody
conjugate alone L. (45/NP) are not labelled. Nuclei are stained with Sytox green (blue channel). Outer segment (os); Inner segment (is); Nucleus (n); Synapse
(s): Axoneme (a); Bands (b). Scale bar: 20 mm. |
