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Fig. 4. CARTp-immunoreactivity in the mesencephalon and
rhombencephalon. A: Pretectal region. Note heavy staining of neurons
in the central thalamic nucleus (C). B: Optic tectum. Note stained
cells in layer 9 and intense fiber staining in the periventricular layers
(pvl). C: Immunostained fibers and cells in the optic tectum (tect) and
the magnocellular nucleus (mt) of the torus semicircularis. D: Sagittal
section of the mesencephalon and the rhombencephalon. Note intense
fiber staining in the tectum and fasciculus retroflexus (arrow), and
heavily stained neurons in the central thalamic nucleus (C). E: Intense
fiber staining in the isthmic region. Note that the isthmic (Is)
and the interpeduncular (Ip) nuclei are free of immunostaining.
F: Stained cells and fibers in the superior raphe nucleus (r). aq,
cerebral aqueduct; cer, cerebellum; cg, central gray; Lpv, lateral thalamic
nucleus, posteroventral division; P, posterior thalamic nucleus;
Pn, pretectal neuropil; Pv, posteroventral tegmental nucleus; 4th,
fourth ventricle. For other abbreviations, see list. Scale bar 300 m
in A; 100 m in B,F; 200 m in C,E; 500 m in D. |
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Fig. 5. CARTp-immunoreactivity in the rhombencephalon and the
spinal cord. A: Neurons in the nucleus of the solitary tract (nst,
arrow). B: Intense staining of fiber terminals in the ambiguous nucleus
(IX–Xm, arrow). Note stained cells dorsomedially. C: Neurons in
the gracile nucleus (Ng) of the dorsal column nuclei. D: Fiber terminals
and neurons in the intermediate zone of the spinal cord. Horizontal
section. E: Stained cells (arrows) medial and dorsal to the
lateral motoneurons in the spinal cord in a lumbar segment. Note
fiber staining in the dorsal horn (dh), and among unstained motoneurons
(vh). The midline is to the left. af, anterior funiculus; cc, central
canal; cg, central gray; lf, lateral funiculus; pf, posterior funiculus; sol,
nucleus of the solitary tract; 4th, fourth ventricle. Scale bar 100 m
in A–D; 200 m in E. |
