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Fig. 2. Developmental expression profiles of DHCR7 and Shh. (A) RT-PCR analysis of DHCR7 and Shh expression during Xenopus development. H4, histone H4. (B) Whole-mount in situ hybridization of DHCR7 and Shh at gastrula (a,e), neurula (b-d,f-h,i,j,m,n), tadpole (k,l,o,p) stages. (a,b,d,e,f,h) Dorsal views. (c,g) Anterior views. (i-p) Lateral views with anterior towards the left and dorsal upwards. dl, dorsal lip; fp, floor plate; arc, archenteron; tel, telencephalon; ave, anterior ventral endoderm; ba, branchial arches; fnr, frontonasal region; age, presumptive anterior gut endoderm; nd, notochord; ov, otic vesicle. (C) Induction of DHCR7 by activin in animal cap explants. CHX, cycloheximide; WE, whole embryos. |
