| |
Figure 4. Xenopus tropicalis Gata6. Whole-mount in situ hybridization analysis of tGata6 expression in tropicalis embryos. A vegetal view of stage (st.) 10 (A) and stage 11 (B) gastrula embryos show tGata6 transcripts localized to the deep endoderm but absent from the superficial endoderm (red arrow). C: A midsagittal hemisection of a stage 10.5 gastrula previously stained by whole-mount (dorsal lip to the left) shows tGata6 transcripts abundant in the leading edge endoderm and confirms the lack of expression in the superficial endoderm (red arrow). A tGata6 whole-mount of a stage 22, early tail bud embryo(D) and a midsagittal section of the same embryo (E) is shown with anterior to the right. ld, liver diverticulum; cm, cardiac mesoderm; lpm, lateral plate mesoderm. F: A section of a stage 26, whole-mount embryos shows tGata6 expression in the endoderm and the cardiac mesoderm. G: By stage 32, tGata6 is strongly expressed in the mid-gut endoderm as well as the cardiac and lateral plate mesoderm. |
