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Fig. 2.
AChE is required for endoderm cell rearrangement and polarization. In NF 46 intestine sections of embryos injected with control MO (A), AChE MO (B), AChE MO plus wt AChE mRNA (C), or AChE MO plus mutAChE mRNA (D), β-catenin (red) outlines membranes of injected (GFP labeled, green) and uninjected cells. Serial sections from embryos in A-D were immunostained for integrin (green) and aPKC (red) (E-H); the boxed regions are shown at higher magnification in I-L. A single-layer columnar epithelium of polarized cells forms in control MO-injected intestines (I). AChE MO-injected cells (J) are rounder (asterisks), unpolarized [absence of aPKC (red), arrowhead] and fail to form a single layer. Defects are rescued by co-injection with wt AChE mRNA (K) or mutAChE mRNA (L). Scale bars: 100 μm in A-H; 25 μm in I-L. |
