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Fig. 5. Xmad4 expression profile by whole-mount in situ hybridization. In all panels, anterior is to the left. A: Dorsal view of a late neurula embryo showing strong Xmad4 expression in the ectoderm immediately adjacent to the neural tube. B: Anterior lateral view of an early tailbud stage embryo. Note expression in the Y-shaped hatching gland (black arrow) and the more ventral cement gland (white arrow). C: Lateral view of a mid-tailbud stage embryo showing Xmad4 expression in the pronephros (black arrow). The darkness of the head is background, due to the extended period of staining required to visualize the relatively low level of transcription in the kidney. D: Close-up view of a mid tailbud stage embryo. Xmad4 expression is detected in the embryonic liver (arrow). |
