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Figure 4. PRC1 is Dynamically Expressed in the Developing Xenopus Embryo(A) In situ hybridization of PRC1 mRNA in an early-neural-plate-stage embryo. Arrowheads outline the border of the neural plate.(B) In situ hybridization of PRC1 mRNA in a late-tail-epidermis-stage embryo. Arrowheads show areas of high expression of PRC1 mRNA, including the brain, kidney, and tailbud.(C) Immunostaining of PRC1 in the tailbud epidermis. White arrow indicates the line scan indicated by the pink line in (E). Red arrowhead indicates a midbody stained by PRC1. Scale bar represents 20 μm.(D) Immunostaining of PRC1 in the neural plate. White arrow indicates the line scan (indicated by the blue line in (E). Red arrow indicates a midbody stained by PRC1. Scale bar represents 20 μm.(E) Graph of line scans for PRC1 signal intensity in representative tailbud epidermal cells (pink) and neural-plate cells (blue). Arrow and arrowhead indicate the intense signal at midbodies in both cell types.(F) Graph of the mean pixel intensity of interphase, cytoplasmic PRC1 immunstaining signal for tailbud epidermis cells (pink bar; n = 12 embryos) and neural-plate cells (blue bar; n = 7 embryos).(G) Immunostaining for PRC1 (green) and α-tubulin (red) in a tailbud epidermal cell at early telophase. PRC1 channel alone is shown in (G′); PRC1 is concentrated around bundled microtubules in the midzone. Scale bar represents 5 μm.(H) Immunostaining for PRC1 (green) and α-tubulin (red) in a neural-plate cell at early telophase. PRC1 channel alone is shown in (H′); despite cytokinesis onset, PRC is not concentrated in the midzone. Scale bar represents 5 μm.(I and J) By late telophase, PRC localizes to the central spindle in both epidermal cells (I, I′) and neural cells (J, J′). |
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prc1 (protein regulator of cytokinesis 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 27, lateral view, anterior right, dorsal up. |
