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FIG. 4. (A) Fluorescence micrographs of sperm binding to an oocyte, coelomic egg, dejellied oviposited egg, and fertilized egg (0.5 hr after
fertilization). The sperm heads were stained with Hoechst 33342 (0.2 mg/ml) and appeared in the images as bright dots on the surface of
the top, darker animal hemisphere the egg. The vegetal hemisphere appeared brighter due to autofluorescence. Because of the size of the
egg (1.2–1.3 mm in diameter), not all of the sperm are in focus. Bar, 0.2 mm. (B) Fluorescence micrographs showing polyclonal anti-gp69/
64 antibody staining of an oocyte, coelomic egg, dejellied oviposited egg, and a dejellied fertilized egg. The vegetal hemisphere appears
brighter due to autofluorescence, not because of a higher level of staining by the antibody. See Materials and Methods for details. |
