Summary Attributions

Clone Name: IMAGE:8536363	Gene: twist1.L	Species: Xenopus laevis

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Sequence:

Full length sequence: BC129769 [+]

CTTGGAGAAGACTTGACACGGGACATCTGTAGGGACAGAGCCTGCCCTCTGCCCGGGACCTTCTCCTGACTGTGCCGGGAGAAAATGATGCAGGAAGAGT
CCAGCTCGCCAGTGTCCCCGGTAGACAGTGCGAGTAACAGCGAGGAAGAGACTGACAGACAGCAGAGCAAGAGGGGATGCAGGAAGAAGCGAGCCACTAG
GAAAAGCCCCGAGGATCCCGACAGTCCGAGCTCAGTGAAGCGCAACAAGAAGGCGAGCAGCACGGGCAGTAGCCCCCAGTCCTTTGAGGAGCTGCAGAGT
CAGAGGGTCATGGCCAATGTCAGGGAGCGTCAGAGGACTCAGTCCCTCAATGAAGCCTTCGCAGCCTTGAGAAAGATCATCCCCACCCTCCCCTCGGACA
AACTGAGTAAAATCCAAACGCTCAAACTGGCCTCCAGATACATCGACTTTCTCTGCCAGGTCTTACAGAGCGACGAGCTGGACTCCAAAATGGCCAGCTG
CAGTTATGTGGCCCATGAGAGGCTCAGCTATGCCTTCTCCGTGTGGAGGATGGAGGGAGCGTGGTCCATGTCTGCATCTCACTAACAGCAGTGCCACTAC
AGCTCAGGCCACACACACAGACAATACTTTTACCAGTGAATGGGAAAATGGACAGTGTAGAAACTGGAGCTGGATATCCCAAACAATTAAATCTGAGGAT
TTTTGTGTGTTAGGGTGAAAGGGCCGAAGCGAATGAAGGCGTGCGGAGCACTTACAGAGCAAATGTGCCCTGGGACACTCAGTCTATACAACAGGGAGCC
GTGTAGGTTCGCCAAATCCATTCCATTCTGATCCTGATCAAATCTCTCATCTGGACTTTGACGAAGAATGTTGGAAATTATTATTTTTTTAATTCATTTT
CCTTGTACTTTTTTTTTATATACTGCATGCATGCGTTACCACCAGTCGTGCCTTTGAAGCCACTTAAGAGGAAAATATTTAAACATTCCCATTCTTGAAG
GTTTCTGTCGAAATCCATTTTTTTTTTTTAAAAATAGGAAGGACTATTTTTTTTTCTATTTGATTCTTGGTTCAGAAAACTGTTTCTATACAAGCGGCGG
CATCTCTTTATTCATCACCTGAGAAATTTATTTATTTATTGATCCATGTTTAAAAAAAAAAAAAAAATGCAACTAGATCCGGTGTCTAAATGCATTCATA
TTTTTTATGGTTGTTTTGTAAATATCATTGTATATTATTTTTCTGCAATAAATAAATATGATTGAAAAAAAAAAAAAAAAAAAAAAAAAA

5' EST: EB473667 [+]

aaaannnccatcgtttnnngaggagtcccgattaaattcgtccccttggagaagacttgacacgggacatctgtagggacagagcctgccctctgcccgg
gaccttctcctgactgtgccgggagaaaatgatgcaggaagagtccagctcgccagtgtccccggtagacagtgcgagtaacagcgaggaagagactgac
agacagcagagcaagaggggatgcaggaagaagcgagccactaggaaaagccccgaggatcccgacagtccgagctcagtgaagcgcaacaagaaggcga
gcagcacgggcagtagcccccagtcctttgaggagctgcagagtcagagggtcatggccaatgtcagggagcgtcagaggactcagtccctcaatgaagc
cttcgcagccttgagaaagatcatccccaccctcccctcggacaaactgagtaaaatccaaacgctcaaactggcctccagatacatcgactttctctgc
caggtcttacagagcgacgagctggactccaaaatggccagctgcagttatgtggcccatgagaggctcagctatgccttctccntgtggaggatggagg
gagcgtggtccatgtctgcatctcactaacagcagtgccactacagctcaggcccacacacagacaatacttttacantgaatgggaaatggaagtgtag
aactggagctgattcccaacaataatctgagatttttgtgtaggtgaaaggcgaacaatgagctgcgacctacaagcatggcctggaccatctacaagag
cggagtgcaaccatcttgacgtaattcatcgattgaaagtggattttttatcttctcttttat

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Library:

Name: NICHD_XGC_limb_m

External Dbs: Unigene laevis cDNA library, Xenopus IMAGE cDNA Library

Description: cDNA was primed using oligo-dT primer: GACTAGTTCTAGATCGCGAGCGGCCGCC(T)25 and cloned into the SmaI/NotI sites of pCS111. Size-selection >1.25 kb resulted in an average insert size of 1.6 kb. This primary library was constructed by Express Genomics (Frederick, MD). Note: this is a Xenopus Gene Collection library.

Anatomy: limb	Stage: NF stage 54 to NF stage 61
Normalized: No	Subtraction: No

Vector Details	Vector Map
Name: pCS111 Type: plasmid 5' Restriction Site: SmaI 3' Restriction Site: NotI

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Usage:

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Suppliers:

Search for clone at: Open Biosystems