XB-CLONE-1681322
|
Clone Name: XL470i16ex
|
Gene:
ubc.L
|
Species: Xenopus laevis
|
|
|
|
Sequence:
5' EST: BP693425 
[+]
aactgattgaatctcgcgcaggacccgctcgtcagcaaacatgcagatatttgtgaaaactctcactgggaagaccatcacacttgaagttgagccaagt
gacacaattgagaatgtgaaagctaaaatccaagataaagaaggcattccccctgaccaacaaaggttgatcttcgctggcaaacagctggaggatggac
gaaccctgtcagattacaacattcaaaaggaatccactctgcacttggttctccgtttgagaggggggatgcagatatttgtgaagaccctgacagggaa
gacaattactcttgaggtggagccaagtgacactattgagaatgtaaaggcaaaaatccaagacaaagaaggtattccccctgaccagcagagattgatt
tttgctggtaagcagctggaagatggcagaaccctgtcagactacaacattcagaaagagtccaccttgcacttagttcttcgtctcagaggtgggatgc
aaatatttgtgaagaccctgacagggaagacaattactcttgaagttgagccaagtgacactattgagaatgtaaaggccaaaatccaagacaaggaagg
cattccccctgatcaacagagattgatatttgctggcaaacaattggaagatggaagaaccctgtcagactataatattcagaaggaatctactctgcac
ttggtccttcgtctcagaggtggcatgcagatatttgtcaagaccttaactgggangaccattacccttgaagttgaaccaagtgacaccattgaaaatg
taaaagcaaaaatccaagacaaagaaggcattccctcctgatccncanaagattgnatttt
3' EST: BP727603 [+]agtaatgcagagcagatgcataaaaggattgtcttaaatcccacccctcaaacgcaggaccaggtggagggtggattctttctgaatgttgtagtcagag
agagtgcggccatcttccagctgcttgccagcaaatatcaatctctgctggtcagggggaatgccttccttatcctggatttttgcctttacattctcaa
tggtgtcacttggttcaacttcaagagtaatggtcttcccagttaaggtcttgacaaatatctgcatcccacctctgagacgaagaactaagtgcaaggt
ggactctttttgaatgttgtaatcagaaagagtgcggccatcttccagctgcttcccagcaaaaatcaatctctgttgatcagggggaatgccttctttg
tcttggatttttgccttaacattctcaatggtgtcacttggttcaacttctagagtaattgtcttcccggncagtgttttcacaaatatctgcatcccac
ctctcagacgaagaactaagtgcaaggttgactctttctgaatattgtagtcagacagggttctgccatcttctagttgcttaccagcaaaaatcaatct
ctgctggtcagggggaatgccttctttgtcttggatttttgcttttacgttntcaattgtgtcacttggttcnacttcaagagtaattgttttaccagtn
aaggtcttgacaaaatatctgcntcccncctcngnagacgaaagaactaaagnggcaangtggattcnttnttnaatgttgtnantcagnaaagaagngc
ggnccatcttccanctgcttaccnacccaaaacnaactctctnctgatc
Library:
Description: About 500 pieces of anterior neural plates were dissected from stage 12-12.5 embryos. The neuroectoderm layer was separated from the underlying mesendoderm layer in 1 x modified Barth's solution containing 1-2 mg/ml collagenase. After poly (A)+RNA selection by an oligo(dT) cellulose column, a cDNA library was made with the SuperScript plasmid system for cDNA synthesis and plasmid cloning (Invitrogen) and the pCS105 vector (Hsu D.R. et al., Mol Cell. 1, 673-83, 1998). The first strand cDNA was synthesized with oligo dT-NotI primer. After second strand synthesis, SalI adapter (gtcgacCCACGCGTCCG; the lower case letters show SalI site) were ligated and the cDNA was directionally cloned with SalI at the 5' end and NotI at the 3' end. The average insert size is about 2kb and the host bacteria is XL1 blue (Stratagene) (?).| Vector Details | Vector Map |
Name: pCS105
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
