XB-CLONE-1720230
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Clone Name: XL492m24ex
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Gene:
ubc.L
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Species: Xenopus laevis
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Sequence:
5' EST: BP707891 
[+]
acaattactcttgaggtggagccaagtgacactattgagaatgtaaaggcaaaaatccaagacaaagaaggtattccccctgaccagcagagattgattt
ttgctggtaagcagctggaagatggcagaaccctgtcagactacaacattcagaaagagtccaccttgcacttagttcttcgtctcagaggtgggatgca
aatatttgtgaagaccctgacagggaagacaattactcttgaagttgagccaagtgacactattgagaatgtaaaggccaaaatccaagacaaggaaggc
attccccctgatcaacagagattgatatttgctggcaaacaattggaagatggaagaaccctgtcagactataatattcagaaggaatctactctgcact
tggtccttcgtctcagaggtggcatgcagatatttgtcaagaccttaactgggaagaccattacccttgaagttgaaccaagtgacacaattgaaaatgt
aaaagcaaaaatccaagacaaagaaggcattcctcctgatcagcagagattgatttttgctggtaagcagctggaagatggccgcactctttctgactac
aacattcaaaaagaatccaccttgcacttagttcttcgtctcagaggtgggatgcagatatttgtcaagaccttaactggtaaaacaattactcttgaag
ttgagccaagtgacactattgagaatgtcaaggctaaaatccaggataaggaaggaattcctccctgaccaaccaaagattgatcttttgcaggcaagca
nctgggaagatggccngcnctctctctgactacaatattccanaaagaatccnctctgcantttgggtccnttcgtctcanaaggngggaatgcaaaata
tttgtcaagacctttanctggg
3' EST: BP739884 [+]tgcacaagcaagtaatgcagagcagatgcataaaaggattgtcttaaatcccacccctcaaacgcaggaccaggtggagggtggattctttctgaatgtt
gtagtcagagagagtgcggccatcttccagctgcttgccagcaaatatcaatctctgctggtcagggggaatgccttccttatcctggatttttgccttt
acattctcaanggtgtcacttggttcaacttcaagagtaatggtcttcccagttaaggtcttgncaaatatctgcatcccacctctgagacgaagaacta
agtgcaaggtggactctttttgaatgttgtaatcagaaagagtgcggccatcttccagctgcttcccagcaaaaatcaatctctgttgatcagggggaat
gccttctttgtcttggatttttgccttaacattctcaatggtgtcacttggttcaacttctagagtaattgtcttcccggtcagtgttttcacaaatatc
tgcatcccacctctcagacgaagaactaagtgcaaggttgactctttctgaatattgtagtcagacagggttctgccatcttctagttgcttaccagcaa
aaatcaatctctgctggtcagggggaatgccttctttgtcttggatttttgcttttacgttttcaattgtgtcacttggttcaacttcaagagtaattgt
tttaccagttaaaggtcttgacaaatatttgcatcccacctctgagacgaaaggaccaaatgcaaagtggattcnttcctgaatatttgnagtcagagaa
gagtgccggccatcttccaacctgcttgcccngcaaaa
Library:
Description: About 500 pieces of anterior neural plates were dissected from stage 12-12.5 embryos. The neuroectoderm layer was separated from the underlying mesendoderm layer in 1 x modified Barth's solution containing 1-2 mg/ml collagenase. After poly (A)+RNA selection by an oligo(dT) cellulose column, a cDNA library was made with the SuperScript plasmid system for cDNA synthesis and plasmid cloning (Invitrogen) and the pCS105 vector (Hsu D.R. et al., Mol Cell. 1, 673-83, 1998). The first strand cDNA was synthesized with oligo dT-NotI primer. After second strand synthesis, SalI adapter (gtcgacCCACGCGTCCG; the lower case letters show SalI site) were ligated and the cDNA was directionally cloned with SalI at the 5' end and NotI at the 3' end. The average insert size is about 2kb and the host bacteria is XL1 blue (Stratagene) (?).| Vector Details | Vector Map |
Name: pCS105
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
