XB-CLONE-2042654
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Clone Name: gfi1b-859
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Gene:
gfi1b.L
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Species: Xenopus laevis
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Description: EXRC Clone Number 859.
Xenopus laevis growth factor independent 1B transcription repressor.
Image EST 5506836, Accession BC077878.
Full Length.
To generate probe for in situ.
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Sequence:
Full length sequence: BC077878 
[+]
CAACTGCCAGCCAAGGAGCAGACAGACAGACAGCACTCACTACCCTACTCTGCTGAACTGAGAAAATGCCGCGATCTTTTTTGGTAAAAAGTAAAAAAAC
GCACACATACAACCAGCATCGCTATGTAGAAGAGCAGCCAGTCACTGGAGTCGACCTAGTGGCAACAGTCTACCATGCTCATTGCACGGAATGTCCAGCG
GAATTCCCGGCTTTTAGCACTGACCCTACCGAAAAACAGCATACGCCTGAAAATGTCATAACAGAGGAGGCGAGAAGTGATCCAGGAGATCCAAGAGAGA
AGATCCCATTCTTAAGTAGAAGCATCTCCCCAAATTCATTCCAAGGAATAGCCCTGTCTTCAATGAGACCTTTAAAACACTATGACCCCAGCCCTCTGTC
TGCCTTCTACACACAGAATTTCTCCTGGGATGCTCTGCACTCCACTTATGGCTTCAAGCAGATACCATCCCACATACACCCCTCCATGCTCCAGAACTCC
ATCAACTTGTATAGCTGTCCCCCAAGAGTGGACTCAGACTCAGACCATCCTATCAATTATAGCATGAGTTACTCTCCAAAAATGGATACCTACCACTGTG
TGAAGTGTAGCAAAGTGTTCTCCACTTCACATGGCTTGGAGGTTCATGTTCGAAGATCTCACAGTGGAACGCGACCATTTGTATGTAACATCTGTGGGAA
ATCCTTTGGGCACGCAGTCAGCTTGGAACAGCATCTAAATGTCCACTCCCAGGAGAGAAGTTTTGAGTGCAAAATGTGTGGAAAGACTTTTAAACGTTCC
TCCACATTGTCTACCCACCTCCTCATCCACTCAGACACACGGCCATACCCCTGCCAGTTTTGCGGCAAGAGATTCCACCAGAAGTCTGACATGAAGAAGC
ATACTTATATCCATACAGGTGAGAAACCTCACAAATGCCAAGTTTGTGGAAAGGCTTTCAGCCAGAGCTCCAACCTTATCACCCACAGCCGCAAGCACAC
CGGCTTCAAGCCTTTCAGCTGTGACTTGTGCTGTAAAGGATTCCAACGCAAAGTGGATCTTCGACGACACAGAGAGAACCAGCATGGATTGAAATGATGA
TGATGATTTCTTTTCATACATCTCTGCCTATACCGATGTCACTGGACCGGAGATGTTTTTAAGAATACATCTCAATATGATGCTTTGTGAAAGGCCTGTG
ATTGAAAGCTGTCCATGCCAAGAGTTTCTGCAGCTGACTTGGAGGATCTTTGTCATAGACAAAAGCCACCACACTTTTCAACCAATCAGAAACACAATAA
AGCAACAATGAAGAATATGAACTGGGTTACGCAACCATCGCCTGACTGAATGTGATTGGTAGTTTGAGCACATATACACCTACTGAGCCATAGTGTGCCT
TAAAAATAGCTTTTGTATATTTGATAATATAACTGATTTGTATATATTATTTTCTTAATGCTCTCTATGTTAAAAAAATATGAATAAATCAATATTGTTC
TCCATTAAAAAAAAAAAAAAA
5' EST: CF284644 [+]ggattcgtcgacccacgcgtccgcaactgccagccaaggagcagacagacagacagcactcactaccctactctgctgaactgagaaaatgccgcgatct
tttttggtaaaaagtaaaaaaacgcacacatacaaccagcatcgctatgtagaagagcagccagtcactggagtcgacctagtggcaacagtctaccatg
ctcattgcacggaatgtccagcggaattcccggcttttagcactgaccctaccgaaaaacagcatacgcctgaaaatgtcataacagaggaggcgagaag
tgatccaggagatccaagagagaagatcccattcttaagtagaagcatctccccaaattcattccaaggaatagccctgtcttcaatgagacctttaaaa
cactatgaccccagccctctgtctgccttctacacacagaatttctcctgggatgctctgcactccacttatggcttcaagcagataccatcccacatac
acccctccatgctccagaactccatcaacttgtatagctgtcccccaagagtggactcagactcagaccatcctatcaattatagcatgagttactctcc
aaaaatggatacctaccactgtgtgaagtgtagcaaagtgttctccacttcacatggcttggaggttcatgttcgaagatctcacagtggaacgcgacca
tttgtatgtaacatctgtgggaaatcctttgggcacgcagtcagcttggaacagcatctaaatgtccactcccaggagaagaagtttgagtgcaaatgtg
tgaaagactt
Library:
Description: Cloned unidirectionally. Primer: Oligo dT. Average insert size 2.4 kb. Constructed by Life Technologies. Note: this is a Xenopus Gene Collection library.| Anatomy: spleen | Stage: |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
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Whole mount in situ hybridization:
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Expressed on VBI.
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Probes:
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| Type |
Size |
Promoter |
Linearization Site |
Description |
| antisense |
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T7 |
SalI |
SmaI or EcoRI can also be used. |
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Suppliers:
Search for clone at:
EXRC
