XB-CLONE-2080744
Clone Name: IMAGE:6877307
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Gene:
pde9a.S
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Species: Xenopus laevis
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Sequence:
Full length sequence: BC099362 [+]
GAGGATGGACAGTGACATGAAGCAGAGCAGTGAGACACCAAGCAACATCCCTACATCAAAGAGCAACCAACAAATAACCCAGCCTTCAGAAGAGACCCGA
AAGGCCCTAAAACAACCAACATTCAATGTGTGGGGGATGGAGAGGAAACAGATGCTGTGTCTCTTGGAGCAAATGTTTTATGAATTGGATTTGGTGACAG
AGTTTAAGATGGAGCCTGAAACTGTGAGATGCTTCTTGACTTCTGTTCAGAAACATTACCAGGAAAACCCCTTCCACAATTTCTACCATGGATTCTCTGT
CACCCAGATGATGTATTGTGTGATATCCCAGTGCCAGCTGCAGGAACACCTGTCACATATAGATATTCTCACTCTGATGGTGGCTGCCCTCTGCCATGAC
CTGGACCACCCTGGTCTCAGCAATTCATACCAGGTCAACGCTCAGACGGATCTGGCCAGGCAGTACCACAACAAGTCTCCGCTAGAGAACCATCACTGGG
CAGTTGCGTGGCGCATCCTAAGCCAAAAGCAGTCCAATCTGTTACTTGGAGCAGACCCCAAGCAAGTCCCCCACATACAGCAGGAGATCATGGAACTGAT
TTTGGCCACAGACATGGCACATCATGGGGAAATCCTACAGACTCTTCAAAATATAGAGAATTTCAACTTCTCTAACAGAGAGCACGTAACTGCGCTGAAG
AAAGGTCTCATTAAGATCTGTGACATTTCCAATGAAGCTCGGCCAGCAGAACAGGCAGAAATCTGGGCAGATGCCCTCATGGAAGAGTATTTTCTGCAGA
GTGATCGGGAAAAGGATGAGGGTCTCCCAGTGACCCCCTATATGGACCGGGACAAAGTAAAGAAAGCCGATGCCCAGAGTTCCTTCATCAGCTTCCTACT
TATCCCCCTGTGTGAGGCGCTTTGCAAGCACTTCCCTCTGCTGAACAACTTACTGCTGCAACCCCTGAGAGCTGCCAGACTGCGCTACCAACAGCAGATG
GACAATGGGATGCTTGAGAGGCAGGACATGTGACACCGCGGGGCAAGTAGCCGTGATACACGTTACTGACATAACACCTGTGTGGGCGCATGATTCATAG
ATACACTACGAGGGCAGGTATTGCCCATAGAGTCCCTGTGAGGGCAGGAATCAATGACTATAGGGGAAGATATTGCTTCTATAGTCGCCATGAGGCAAGT
ACTGTGAGAAGAGCCTGAGCTGTGGTTTCTTCTCAAACAGACGCATAAAGATAATAGGGTCCAAACTACCAATCAATAAATGATCTGTGTCCCTCCCACG
TGGAGCCACTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CB206128 [+]gactcctatagggcgctagctcgcctcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggccgcataacttcgtatagcatacattatac
gaagttatcagtcgacggtaccggacatatgcccgggaattcggccattacggccgggggaggatggacagtgacatgaagcagagcagtgagacaccaa
gcaacatccctacatcaaagagcaaccaacaaataacccagccttcagaagagacccgaaaggccctaaaacaaccaacattcaatgtgtgggggatgga
gaggaaacagatgctgtgtctcttggagcaaatgttttatgaattggatttggtgacagagtttaagatggagcctgaaactgtgagatgcttcttgact
tctgttcagaaacattaccaggaaaaccccttccacaatttctaccatggattctctgtcacccagatgatgtattgtgtgatatcccagtgccagctgc
aggaacacctgtcacatatagatattctcactctgatggtggctgccctctgccatgacctggaccaccctggtctcagcaattcataccaggtcaacgc
tcagacggatctggccaggcagtaccacaacaagtctccgctagagaaccatcactgggcagttgcgtggcgcatcctaagccaaaagcagtccaatctg
ttacttggagcagacccccaagcaagtcccccacatacagcaggagatcatggaactgatttttggcccagacatggcacatcatggggaaatcctacag
actcttcaaatatagagaaatttcacttctctaacagagaacacgtaactgcccctgaagaagggtctcatttaagatcttgtgaaatttcccatggaag
cttcggcccagcagaacagggcaaaaatctggggcaagaaggccctccagggaagagaaattttcctgcaaagtg
3' EST: CB756314 [+]caaggcggccgacatgttttttttttttttttttttttttttttttctgagtggctccacgtgggagggacacagatcatttattgattggtagtttgga
ccctattatctttatgcgtctgtttgagaaaaaaccacagctcaggctcttctcacagtacttgcctcatggcgactataaaagcaatatcttcccctat
agtcattgattcctgccctcacagggactctatgggcaatacctgccctcctagtgtatctatgaatcatgcgcccacacaggtgttatgtcagtaacgt
gtatcacggctacttgccccgcggtgtcacatgtcctgcctctcaagcatcccattgtccatctgctgttggtagcgcaatctggcagctctcaggggtt
gcagcagtaagttgttttcccaaagggaagtgtttgcaaagcgcctcacacagaggggataagtaggaagctgatgaaggaactctgggcatcggttttt
cttaatttgttccggaccataaagggggtcattgggaaaaccttctcctttttccgattatttcggcaaaaatacctttccatgaagagatctgcccaaa
ttttcttccttgttttgtgggcagaactttctttgaaaatggccccaatccttaattgaaaccctttttttaacggcattaacgggctcctcttgttaaa
aaaaattgaaaattccccctatattttcnaaaaaaaacgtggagaatattccccaggaaggggcccaggatatgtggggcaaaatacagtttccgataat
ttcctccgccattagggtgggggatattgttcgttgggggttcgcttcttgcgttaagataaattggcacgtccttctttgcggttaaagaaga
3' EST: CB755842 [+]ttatctttatgcgtctgtttgagaagaaaccacagctcaggctcttctcaagtacttgcctcatggcgactatagaagcaatatcttcccctatagtcat
tgattcctgccctcacagggactctatgggcaatacctgccctcgtagtgtatctatgaatcatgcgcccacacaggtgttatgtcagtaacgtgtatca
cggctacttgccccgcggtgtcacatgtcctgcctctcaagcatcccattgtccatctgctgttggtagcgcagtctggcagctctcaagggttgcagca
gtaagttgttcagcagagggaagtgcttgcaaagcgcctcacacagggggataagtaggaagctgatgaaggaactctgggcatcggctttctttacttt
gtcccggtccatatagggggtcactgggagaccctcatccttttcccgatcactctgcaaaaaatactcttccatgaaggcatctgcccagatttctgcc
tgttctgctggccgaacttcattggaaatgtcacagattttaatgagacctttcttcagcgcagttacttgctctctgttaaaaaaaattgaaattctct
ctatttttgaaaaaactggtaggaatttccccctgatgtgccatggtctgggggccaaaatcaagttcccatgatctccctgcctggtatgttgggggga
acttgccttgggggtcctgcctcccaaataaacaaaaattgaaaatggcttttttgggcttataagaaagaggcccaaggaaaacatgtccccacgtgaa
taggatttcccccaccccgaaaaacacttagttgtgggggaagtgggcaccgagccccaaatcccgatctgaaaaccgttttaaccctgtgaattaaaaa
tag
Library:
Description: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.6 kb (range 0.9-3.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a Xenopus Gene Collection library.Vector Details | Vector Map |
Name: pDNR-LIB
Type: plasmid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
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