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Summary Attributions
XB-CLONE-2105298

Clone Name: IMAGE:6881085 Gene: acta2.L Species: Xenopus laevis  
 

Sequence:

5' EST: CB208288 [+]

3' EST: CB562438 [+]

Library:

Name: NICHD_XGC_Tad1External Dbs: Unigene laevis cDNA library, Xenopus IMAGE cDNA Library

Description: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.6 kb (range 0.9-3.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a Xenopus Gene Collection library.

Anatomy: whole organismStage: NF stage 54 to prometamorphosis stage
Normalized: NoSubtraction: No

Vector DetailsVector Map
Name: pDNR-LIB

Type: plasmid

5' Restriction Site: SfiI

3' Restriction Site: SfiI		Expression Image

Usage:

Suppliers:

Search for clone at: Open Biosystems