Summary Attributions

Clone Name: IMAGE:6881248	Gene: acta2.L	Species: Xenopus laevis

---

Sequence:

5' EST: CB208422 [+]

ggggcaccatcccggagtagcaaccctagcaaagcaccgagagggtttcttgttggaaaggttttagcaagtagcaatacaagatgtgtgacgaagaaga
cactactgcactggtgtgcgacaatggctctggactctgcaaggctggatttgctggggacgatgctccaagggccgtattcccctccatcgtgggtcgc
ccaaggcaccagggtgtgatggttggtatgggtcagaaggacagctatgttggtgacgaagctcagagcaagagaggtatcctgacactgaaatacccaa
ttgaacacggcatcattacaaactgggatgatatggaaaagatttggcaccacagcttctacaatgaattacgagtggccccagaggaacacccaaccct
gctcacagaggctccactgaatcccaaagcaaacagagaaaaaatgacccagataatgtttgagacattcaacgtgccagcaatgtatgttgctattcag
gcggtgctatccctctacgcttctggccgtactactggtatcgtacttgactctggtgatggtgtcacccacaacgtaccaatctatgaagggtatgctc
tccctcatgcaatcatgcgtcttgacctggcttggcgtgacctaacagactacctcatgaagattttgactgaacgcggctattcctttgtaactactgc
tgagcgtgaaatcgtgagagacatcaaggagaagctgtgctacgtaaccccttgacttg

3' EST: CB561264 [+]

gtgatcccatctgctggaaggtagacagggaggcgaggatggagccaccaatccagacggagtacttgcgctcaggtggggcaatgatcttgattttcat
tgtgctgggagccaaagctgtgatctccttctgcattctgtcagcaatgccagggtacatggtggtaccaccggacaatacgttgttggcatacaggtcc
tttcggatgtctatgtcgcatttcatgatgctgttgtaagtggtttcatggatgccagcagattccataccaatgaaggatggctggaacagggtctcgg
ggcagcggaaacgctcatttccaattgtgatgacctgaccgtcgggaagttcataactcttctctagagaggaggaagaggcagcagtagccatttcatt
ctcaaagtcaaaggctacgtagcacagcttctccttgatgtctctcacgatttcacgctcagcagtagttacaaaggaatagccccgttcagtcaaaatc
ttcattgagtagtctggtaggtcacgggccacccaggtcaagacgcatgattggctgaaggaaaaccaaccccctcataaattggtacgttgggggggga
caccctccccgaagtcaagtacaaaacccatattacggcccgaaaacgtaaaggggataccaccccccccgaaaaacaaaaaaaaaattgtctggggcca
ctttaaatggtttcaaaaaaattatattcgggagcaaatttttttcccccgttttggcctttggggaaattcagcgggggaaccccccttgtaaaaacaa
gggtatgagggggtttccccccctggggggggccacccccaagaaatatttaatttggttanaaaaacctctggattggcg

---

Library:

Name: NICHD_XGC_Tad1

External Dbs: Unigene laevis cDNA library, Xenopus IMAGE cDNA Library

Description: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.6 kb (range 0.9-3.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a Xenopus Gene Collection library.

Anatomy: whole organism	Stage: NF stage 54 to prometamorphosis stage
Normalized: No	Subtraction: No

Vector Details	Vector Map
Name: pDNR-LIB Type: plasmid 5' Restriction Site: SfiI 3' Restriction Site: SfiI

---

Usage:

---

Suppliers:

Search for clone at: Open Biosystems