XB-CLONE-2506369
Clone Name: IMAGE:8917084
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Gene:
gsn
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC154875 [+]
GGGTGGTGGCCCTGTGGCTAAGCTTTCTGTGCGTGTAGCAGCCCCGCAGCCTCATTCGCTTCTCTCTCTTCAGTGCAGGAGGGACCGCAGCTTGTGTGCC
CAGTGACCCAACAGCCTTCAGAAATGGTGGTTGACCATCCCGAGTTCGAGAAAGCCGGAAAGGCCCCGGGGCTCCAAGTATGGAGAGTGGAGAAATTCGA
TTTGGTGGCCGTTCCTCAGAATCAATATGGATCCTTCTTCACCGGGGATGCCTACCTTGTACTCAGCACCATCAAGACTCGCTCTGGGAACCTGCAGTAC
GATCTTCACTACTGGCTAGGGGATGAGTGCACTCAGGATGAAAGTGGATCGGCTGCCATTTTCACAGTGCAAATGGACGACCATCTAGGAGGACAGCCCA
TCCAGAACCGAGAAGTTCAAGGATATGAGTCCTCCACATTCTTGGGATACTTCAAGCCTGGCATCAAATACAAGGCTGGAGGCGTGGCTTCAGGTTTCAC
GCACGTGGTACCAAACGAGGTAGACATCAAGCGACTCCTCCAGGTGAAAGGCCGCCGTGTTGTTAGAGCCACCGAGGTTCCCGTGAACTGGGACAGCTTC
AATCAAGGAGACTGCTTTATTCTCGACCTTGGGAATGAAATCTACCAATGGTTTGGGTCGAAATCCAACCAGTTTGAGAAACTGAGAGCCACGGCAGTTG
CCAAGGGCATCCGGGACACGGAAAGGAACGGAAGATCCAAGTTGTATGTGGTGGAAGAAGGGATGGAGAGAGAGAAAATGATTGAGGTTCTGGGACAGAA
GCCAGATTTGCCAGAGGGACCAGCGGATGATATTAAAGCCGATGCTTCCAACAGGAAACTTGCAAAGCTTTATAAGGTCTCTGATGGAAAGGGTGCCATG
ACGGTGTCTCTAGTGGCAGATCAAAACCCCTTCACCCAGGCGGCCCTCAACTCAGATGACTGCTTTGTACTTGATCACGGCTCAGATGGAAAGATCTTTG
TATGGAAAGGAAGGAATCCCAACATGGAAGAGAAGAAAGCTGCACTAAAGACTGCCACTGAATTCATTTCCAAGATGGGCTACCCAAAGCAAACTCAGGT
CCAAGTGTTGCCAGAGAATGGCGAGACTCCCCTGTTCAAACAGTTCTTCAAGAATTGGAAAGACAAGGAGGCCACCGAGGGCATGGGTGTTGCCTACGTC
CCCCACCACATCGCCAAAATTGAAAATGTGCCTTTCGACGTCAGTGGTCTACACGAATCCCCGGCCATGGCTGCCCAGCACGGCATGGTGGATGATGGAT
CTGGCCAAAAACAGATTTGGCGTATTGAAGACTGCAAGAAAGTTCCTGTTTCTAAATCCCTTTACGGCCAGTTTTATGGTGGAGACAGCTACATCATTCT
GTATAATTATAAGCACGGCGGAAAACAAGGCCAGATCATCTACACATGGCAAGGGGACGAGTCGTCAAAGGATGAAGTTACCGCCTCGGCCATCCTGAGC
GCCCAGCTGGACGAGGAGCTTGGCGGGACCCCAGTACAGGTGCGGGTCGTGCAGGGCAAGGAACCGGCCCATCTAATGAGCCTGTTTGGTGGGAAGCCCA
TGATTGTCAACAAAGGTGGAACTTCAAGAGAAGGCGGTCAGACCAAGGACGCGGACGTCCGCCTGTTCCAAGTCCGTGCTTCCTCCTCTGGGTTCAGCAG
AGCAGTAGAGGTGGATTCTACAGCCAGCAATCTGAACTCCAACGATGCCTTTGTGCTGAAGACCCCCTCTGCCGCCTACCAGTGGGTTGGCCAAGGTTCC
ACCGATGCTGAAAAGAAGGGAGCCCAAGAGCTATTGGCGGTGTTGGGAGTCTCTGCGTCTGAGATACTGGAAGGCCAGGAAACTGATGACTTCTGGGCGG
CGCTAGGCGGTAAAGCAGATTACCGTACCTCGGCAAGACTAAAGGACAAGTTGAACACCCACCCCCCTCGTCTGTTTGCCTGTTCCAACAAGACCGGCCG
CTTCATTATTGAGGAGGTTCCAGGAGAAATATCTCAGGATGATCTCGCTACCGACGACGTCATGCTGTTGGACACTTGGGATCAGGTCTTTGTCTGGGTG
GGAAAAGAAGCCCAGGAAGACGAGAAGAAAGAGGCTATAGCATCGGCCTACAAGTATATTGAGAGTGACCCAGCTAATAGGGACAAGAGGACCCCAGTTG
CCATCATCAAGCAAGGATTTGAGCCCCCCACATTCACAGGCTGGTTCCTGGGTTGGGAGACAGATTACTGGGATATCAGCCCCCTGGAGAGAGCGCTGGC
AGACCTTTAATGAGACAAGCCCTTCCCGTGGGTCTCTCTGTGCCTTTACCGACTAGGAGACGCGCTCAGCCAGAGACTGTTCCTAGCCCCACGTAGCTGT
GCAATAACCAAAGTACCCCCCCAGTTTGTACCTCGACCCCCTCTTGTACTATTGGAATATTTAAAAGCAATAAAGCAAACACATGTTCTACAAAAAAAAA
AAAAAA
5' EST: EL830473 [+]gggtggtggccctgtggctaagctttctgtgcgtgtagcagccccgcagcctcattcgcttctctctcttcagtgcaggagggaccgcagcttgtgtgcc
cagtgacccaacagccttcagaaatggtggttgaccatcccgagttcgagaaagccggaaaggccccggggctccaagtatggagagtggagaaattcga
tttggtggccgttcctcagaatcaatatggatccttcttcaccggggatgcctaccttgtactcagcaccatcaagactcgctctgggaacctgcagtac
gatcttcactactggctaggggatgagtgcactcaggatgaaagtggatcggctgccattttcacagtgcaaatggacgaccatctaggaggacagccca
tccagaaccgagaagttcaaggatatgagtcctccacattcttgggatacttcaagcctggcatcaaatacaaggctggaggcgtggcttcaggtttcac
gcacgtggtaccaaacgaggtagacatcaagcgactcctccaggtgaaaggccgccgtgttgttagagccaccgaggttcccgtgaactgggacagcttc
aatcaaggagactgctttattctcgaccttgggaatgaaatctaccaatggtttgggtcgaaatccaaccagtttgagaaactgagagccacggcagttg
ccaagggcatccgggacacggaaaggaacggaagatccaagttgtatgtggtggaagaagggatggagagagagaaaatgattgaggttctgggacagaa
gcca
3' EST: EL830474 [+]tttttttttttttttgtagaacatgtgtttgctttattgcttttaaatattccaatagtacaagagggggtcgaggtacaaactgggggggtactttggt
tattgcacagctacgtggggctaggaacagtctctggctgagcgcgtctcctagtcggtaaaggcacagagagacccacgggaagggcttgtctcattaa
aggtctgccagcgctctctccagggggctgatatcccagtaatctgtctcccaacccaggaaccagcctgtgaatgtggggggctcaaatccttgcttga
tgatggcaactggggtcctcttgtccctattagctgggtcactctcaatatacttgtaggccgatgctatagcctctttcttctcgtcttcctgggcttc
ttttcccacccagacaaagacctgatcccaagtgtccaacagcatgacgtcgtcggtagcgagatcatcctgagatatttctcctggaacctcctcaata
atgaagcggccggtcttgttggaacaggcaaacagacgagggggggtgggtgttcaacttgtcctttagtcttgccgaggtacggtaatctgctttaccg
cctagcgccgcccagaagtcatcagtttcctggccttccagtatctcagacgcagagactcccaacaccgccatagctcttgggctcccttcttttcagc
atcggtggaaccttggccaacccactggtagcggcagagggggtcttcagcacaaaaggcatcgttggagt
Library:
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: