XB-CLONE-2522815
|
Clone Name: IMAGE:8857013
|
Gene:
kif5b
|
Species: Xenopus tropicalis
|
|
|
|
Sequence:
5' EST: EL819799 
[+]
gccagtggctgtccgtggaggcgtggctaaacaagacaaagtctgctgagaggctatcggagcacaggagtcggagaatcgtgcagtgacgttgagaaac
gcacttatctctatgggattgtaggattgtttttgaaattgtaatttatctgacctgtacagctctccttttccctctcctcagtgtctggcaaattcta
actgatttgcggcaaaaaaaactagagtgcaatttcgttgtcctcgctttacaacatgtaatttactatgtagcgccatccattgcacagtttgttatcc
atgtcttaaaaatgtagtctgcactgtgccaagttggaacataagttaccggttagatagacccttgttatattgtgtcagctcttcttctctccggggg
acatggaacaaacgttaagcatttaaacctcttccgggctaagcatttgaaatgcatccctgtttaccgtctcactatattacagcagtgctgacaactc
ctgaagtgacttcatttaatgctacaataaagcaactgtaggttttacactttgcgacagtgtctagatactaatgtgtatactcgctgcagggctcggg
aagtaatatacctttcggaataagaccaggtttccgtgtaatcaaggctgctgttttatctgccggggcacaacacagctgccccgggggttgttggatt
aaataatgtatagtaacaaaatactggagtaaactttttatatacaagcgaatgggatattcatttagcatttgtcctattgcatcacatcctga
3' EST: EL819800 [+]tttttttttttttttgccacttgactttgacatctttattttaaacacaaatacatttacagcatgcagagagtttgaagtaaatggcatttcctctgtc
cagttttcactctgaatgtattggatccaaaccggaaggcaggcagactgtgatctgctgaggcataacccgttagaaagaacgaacaagcagtcagcgt
ttctttaaattaggcaaataatttagttgtttatactgcataaatatttactttttaccccaaatcggtgttgtagggacaaaaaaagaaagaaaataag
gaagagacaatgagtaaaacagtatgtaatagaaaacctaaacagaaaagagaaacccatacaagcaataacatagaataaatgggcaagagactgtaaa
gaagagaggcagagtacagaaaatattccgtatcttaggtttgaggctaatgtttatttaaccattaatggcaaaagttacagattttaatttattagca
agtctgatatcatacaaaggaaatcaaacgagagagcaatgtaggcacacaactgaatggtaatgtacaatgggatatatatctataactgatcaacact
gtcccctgagacaagcagctggaccggcacacagcggataaaaggaaaaattagtgttcaatacaagtgcactgccttcaccatgaactgcacgagcaga
tttgcctgaggtcccggaaccgcaccatctggtctacttcattgcaccgcaatgggttctggga
Library:
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.| Anatomy: testis | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
