XB-CLONE-2522858
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Clone Name: IMAGE:8856961
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Gene:
prmt5
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Species: Xenopus tropicalis
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Sequence:
5' EST: EL819771 
[+]
gttggatgctactatgtacgcaggggtattgagccccatatagggcacccaggcttgggctttcattcaaacgattaggtcccctcgttaaacctactaa
actgaccctgcacactgtacttcgcatccaggataataagctttgtgtatatctgccctgaaatattcatacattgcgggttagcagagaagtgccacca
ttacaaagcgggccctgtaccctgtgcaaagtttgactttctgtgtatgccgatctttcatcctcgctttaaaagggaatttttcaaggaacctgcaaaa
tcgcgcccaggaccacagacccgctcagacttgctgctttctggccgagcctctaatggacaatttggaatctcagacctacgaagtatttgagaaagat
cccgtgaaatattctcagtatcaacaggcagtatataaatgtttgctggacagagtgccagaggaagagaaggagacaaacattcaggttcttatggtgc
ttggagcaggccgggggccccttgtgaatgcctcgctcagggctgcaaagcaagcagaacgcaaaatcaaggtgtacgcagtagaaaagaaccccaatgc
tgtgataacgttggaagggtggcgttatgaggaatggggcagtcaagtgactgtggtgtctggtgacatgagagaatggaaggcacctga
3' EST: EL819772 [+]tttttttttttttttactcaggaaccttttaatattaaacattgctattaacacataataaaaaaaggactataagtgaacaaacatacagttcaactgc
aggaagcctctcatagaggcaaagtcataagtgctgatagtaacccattgtgcgctacccttctgcagcacaaacccctagtatttctccattcgttgta
tgccccatgtcactctcttatgaaggtgatggttttcaaacttgtctatcacaggccaattgtgtaggaacgtcctgtaggattgtgaattgcagagcac
acaggggatgttacagcccattcgtaccaaaccttcttgccattattacaacgccaaaatcggacacaaacagtgtcgccctcccgcaaaggaatgggtt
gcttgataggaaagaggatgggaaaccaagaaaacatgcctggggagtgactttcaggacaaatgctcagcgtcacatctttgtacaacacagcgtcaaa
atatccagcaaacccatgtagcactgtgtttagatctactctgtactgcaggcaaatgtagcggttattatcgattacagcatctttgttggggtggagg
aatgtgaagcatggcaagggatcagataactgatggaagttgtgtagcctaacgacatagggcatctcaaactgag
Library:
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.| Anatomy: testis | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
