XB-CLONE-2525751
Clone Name: IMAGE:8855444
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Gene:
limd1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC166064 [+]
agtgaagccattcagatggtgaagactaaacctaagagatgaacgggccctccatcttctcttttcatttccaggcttaagcgtgtttcttcaagacttc
aatctgctgaatgggagaaagtggcacaaaaagccctgtccaaacatgaatgtacaaaatcgctgctgcaacaaaagatgtcgaaaatggccttgcgtta
cacaaacacaaaatgaacatccaacctccagcaacaccagctacagcacggcaaaatatgacagcagatttggttatgctgtgtgagagactgtaagtca
cagtggtatcgatgtgcagcagagatgaaacaatatcaagtgactgtagtaaaaatgttagcaaaaatgaacaaaactgccccaaaaaatctagaaaagg
aggtaaaacaaccctgcaaatctgtatgtttgtcacaagcaaagtgaaatatttgctgtattacatttagatcaatgcacattgcatcagatttgtgtgt
gtgtgcgtgtgtgtgtgtgcgcgcagagttttgagcagaaattctcttatcttatgaacattaacaagcagatacaatgtaagacattcatttattgact
ataacttgcttgagttaaatgattattggttcttgcataacttgtgctggactagcatcaatgactggtatgttttgtctgtatagccagaatatgacat
tccgtttcacaaggtacaaactgtgtttaaaatgtattatattaatcaacattcataatcatagctaaagtgtagtaactagacttttggtcaaaattga
gttaaagcctaaaatggattagtgacatctgttgaactctcctggttcaattttatcctgattcagaaaaaaaacaagagtgtcaaaattgcagtaacta
caaaacccaagctgatgtaaaaaaaacaaaacaaaacatctgaatggctgcaaccctgcaatgtatagccattatatattatgcataatttatattagtc
ttttcaacagaatgttaatacactttcattgaaatgttttttgattgtgcactgacacaaatacatttaataatacatttcattcatatagctcctataa
atgtacaaaaatggaactgaaaagaaatactatagtacaaaatataatgaaatgttgctataaattaccctcaggcatctaagagttacagcatgtgact
tttttttcttaatgaattgtcgggagccatttatttcaattaagttttgtctgtgtatgtttttttaaactctgaaagtgcgttagccattgccttgtcc
gcagttttggctacaaataatttttaatcttctgttgaagaaacacttcaacagtctcttaaatggattttaatggaattacctttttgactataaaatg
ctttgcattcagtcatgttgcaatataatgtatgaaatcaaaatgtgtttgtaaatgtaattattttctaatgtgataaggattcatatgagtgaagcat
acattatgaattaaattacgcagaaccagatactgatcatatcaggtaatattagtagctttttaaaaaggcctaatggtaatatttgtttgtgaggaga
gccttaaatgttgacgtttttttaaacatgatgtaacttcatttatattatttcccatgagatttaaaggtttatgtgatgactgaaaacgaactttgtg
ttttgttgtttttaaacaatgtctgctatgctttttttttattagggttttttgtacaagaacaaagtttggatggatttgtgtgtgactatgaaataat
tagttgttttcttcagcatttctggaactaatatttgctgatgttatactgataggttgaatttttatgaattgtaataaaaaatgaagaaaacgtgaaa
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa
5' EST: EL817896 [+]gcagctttaaggcaaagacacgatgctgtaaaagaattctacaaatgaatttcatttttgtatggtagcgcaagatttattgtcatgcatatcaagaaat
cggattcattcgagcagatgttcaaagctataatattcttggattgaacagtaaaagttacttgacaaaaattaactaatgactttcatttggttactgg
ctacagagttattttgccattaacagtccttttaacagtttaacactagttttgattttaaatatggatacatatgaggaactaagcctggaagctagca
aattcatacaagatctgactttatatgaagcttctaaagatggactttttcgtgtggataagggagctagtaacaaccctgaatttgaagagaccaaaag
aatatttgctgccaagatggcaaaaatccatcttcagaatcagcagaaaaacattccagacactggcacaagtgaaggaaatggttcaaatctccagtac
acttattcaatgcctaagagtgtagaagaagttccagtcacctataatggtcaatctagttcttcagtaggtcagtctgattacaaaacatacagagagt
cttataaagttaaacctgagattgattctgctgatagttctgaagagcctgtgattcgacatgaacatgaaaccatgttacttagtccagttagtaaagt
tggccatgctattcccagtcaacagtttacttgtaagaaagctatcagtccaagtaatg
3' EST: EL817897 [+]tttttttttttttttgcttgcataactggcagattggcccaccttttattcattaccatgccattcacatcaataataatacattacataaatgtgcaat
ctaaaaactcaactgtacataaatatatttgtattctaaaattacactatccaagcatgtcactttggcagttcaacagaagactcttttcttattctta
gttatgacagattatgattctctaagtattttaaatgacaattatgacaaaacaaatgtccttctagaggataacatcggtggtcatcttcattattcaa
ttccaaagcacaactctcacaacggtaacagtctatgtggtaatctttatccatggacactacacggatagtttcatctgtgccctccgatggcagaatt
ggaaggctgcatgctgcacattttggagctaaaatcttgtggtaatctttgacgcagtagatcttgttctccatgtccacagtgaagggcacaccatcta
aacattcattacaaaccgcacagcggaagcacccaggatgaaatgatttacctagcgcttgtaaaatcatatccatgatccaatgtccacacacaaagca
cctgtctgctgattgatggaatccagaatacaaaaagtcttcttcacagtacactttaccattgacaaaataaaatgcttttcct
Library:
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: