XB-CLONE-2872579
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Clone Name: CABK6277
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Gene:
arf1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT446068 
[+]
cagcaaaggagaggcggcgggaggcgacggaggaacggtggatataaacacactacaatggggaacatctttgccaatctgtttaaaggtctctttggta
agaaggagatgagaattcttatggtgggcttggatgcagccggtaagaccaccatcctgtacaagctaaaacttggcgagatcgtgaccaccatccccac
aataggtttcaatgtggaaacagtagaatacaagaacatcagttttacagtgtgggatgtgggtggtcaggacaaaattcgacctttgtggcgtcattat
ttccagaacactcagggtctgatctttgttgtggatagtaatgatagagagagagtgaatgaagccagagaggagctaacaagaatgctagcagaagatg
agctacgagatgcagtactgttagtgtttgcaaacaagcaggatcttccaaacgctatgaatgctgctgagatcacagacaaactaggcctgcattccct
tcgtcatcggaactggtacattcaggctacctgtgcgaccagtggagatgggctgtatgagggcttggactggctctctaaccagctgaagaatcagaag
tgatcccttcccatataagtgccctccatccctatgcccctctctcccacccaactcccatcctgttcaacatcttggcagagctggccagcacctctgc
tgtctactgggcgggcatgtaatttctttcagcacacattaccgttgtgtgtgtgcttgcacgcctattttgagtgggag
3' EST: DT446067 [+]ttcaatacagaactctttattttagagcatttcagacaaattacaacatggttagaaaacaaaacaaagaaaaaaaaaaaaaaaagatttaggtccaatt
gtacatcatgtacttcacacttgagaatcagtttatacaggaaataaaagttgagctttaaagctttgattcattttaggcatgaagatatgaaaacggt
ctcatttacagacttttaagaagtatatatatatttaaaattgttaagagctacattcccaagtccccacaaaaatggtctacaaatcttttgagtttaa
aaatgaaatggtaccccccttctgctgcacactgaagtaacacaaaatagaagcactctcccacagtgatattaaaaacacattaattgaaatgttcgtt
agttccagaaggcggccttgtgggagggaagcgggggattatgaaggaaaggacagaagcctcagtgtaattggtgagtgtattttgctggtgatacttc
acagcaagccaaatgctgagagaggggataggtcatttacagtaatacatggctctgatgatggcatgggcagccgcttataggactacaactcccagga
tcactctggagtgctgtaggctgccaaactctgccctaacctgtttttattctggctcccatgttctgcttaatagaatggtagccctgtccaacccttt
attttggattcaaatgctatcttccccaaatcaaacaattaactgattatttccttttaaccaaaacccattttcctgcaaccctattttatatattttt
aaactatgttttctctcaaaaacagttgccagttttttttccatataaaattatatatttatatatttatcgagttgtcctacattcttgtta
Library:
Description: The library was prepared from total RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1610 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).| Anatomy: spleen | Stage: |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers:
