Summary Attributions

Clone Name: IMAGE:7786238	Gene: tsc2	Species: Xenopus tropicalis

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Sequence:

5' EST: CX327834 [+]

tcctagatcgaatcccgccctatgacacccacaaaattggtgttctttatgttggagaggggcaggttggcaacgagcgcgcaatcctatccaatgagca
tggctcgtaccgctacacacagttcctcacggggcttgggaagctcatcgagctgaaagacactcagccagacaagatcttcctgggagggttggacggt
tgtggagatgacgggcagttcacctactgctggcatgatgatattatgcaagtcatttttcagatcaccaccctgatgccaaacaaagagatagatccga
accgctgcaacaagaaaaggcacgtgggcaacaactttgtcaatatcatctacaacgactcgggcgagttctacaagttgggcaccctcaagggtcagtt
caactttgtagatgtcaacattcagcctttggactgcgagagcaacctggtgacactgcaatgccgaaaagatatggaaggccttgtagataccagcgtg
gcaaaaattgtgtctgacaaaaacctacccttcctggcccgccagatggctctacattcaaatatacaagaggagttgcaggagctgagtgccaacatgc
cctaccaggtccctactatggccaacaagcccccttcacaaaacccacagactccccccggcgcatttgaaaccggccaaaggacaaggctcatttctgc
tgtggacgatttcacggagtttgcttgatgcacttcaacttgtgcccatggatacgggcttctgatttgatggtacaggcacagccctataaccaccctc
caaactaggaccatggctgtgaagggagggaggaacgcccatcatgaactg

3' EST: CX327833 [+]

cttgagttcagtttattgttaagaagaaaaaaaaacgtgaaaactcattgtgggtctctagagatcctctttgttttccgatgagagcataaataaatta
ggattttaatccaatattgaaaataatttagttttttttagggtgtcctcacaaggtcggaaaagttcattgttaataatcaatggcagttcatgatggg
cgttcctccctcccttcacagccatggtcctagtttggagggtggttatagggctgtgcctgtaccatcaaatcagaagcccgtatccatgggcacaagt
tgaagtgcatcaagcaaactccgtgaaatcgtccacagcagaaatgagccttgtcctttggccggtttcaaatgcgccggggggagtctgtgggttttgt
gaagggggcttgttggccatagtagggacctggtagggcatgttggcactcagctcctgcaactcctcttgtatatttgaatgtagagccatctggcggg
ccaggaagggtaggtttttgtcagacacaatttttgccacgctggtatctacaaggccttccatatcttttcggcattgcagtgtcaccaggttgctctc
gcagtccaaaggctgaatgttgacatctacaaagttgaactgacccttgagggtgcccaacttgtagaactcgcccgagtcgttgtagatgatattgaca
aagttgttgcccacgtgccttttcttgttgcagcggttcggatctatctctttgtttggca

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Library:

Name: NIH_XGC_tropTad5

External Dbs: Unigene tropicalis cDNA library, Xenopus IMAGE cDNA Library

Description: Library constructed using poly A RNA and oligo dT primers (Invitrogen SuperScript Plasmid System for cDNA Synthesis and Cloning). After second-strand synthesis, cDNA was size-fractionated on a sepharose 4B-CL 30 cm column and directionally cloned into pCS108 (http://mcb.berkeley.edu/labs/harland/pages/plasmids.html). Library constructed by Russell B. Fletcher in R. Harland's laboratory (University of California, Berkeley, Dept of Molecular and Cell Biology).

Anatomy: whole organism	Stage: NF stage 35 and 36 to NF stage 41
Normalized: No	Subtraction: No

Vector Details	Vector Map
Name: pCS108 Type: plasmid 5' Restriction Site: SalI 3' Restriction Site: NotI

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Usage:

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Suppliers:

Search for clone at: Open Biosystems