XB-CLONE-3059775
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Clone Name: IMAGE:7847027
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Gene:
ybx3
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT530713 
[+]
cctcgaattaccatctgcatcccaccctccctctccccttcaccctgaaccatctatttcaaatattgggagagaaaaaggggattatacaacatgagtg
aagtaacagctgaagaaccaaagcccaaaacaccaggggctgaagcaagttctccatctgcttcaagtgaaatagaacggaaagtgctggctactaaagt
ccagggaactgtgaaatggttcaatgtgagaaacggctatgggttcattaacaggaatgacaccaaagaagatgtctttgtccaccagacagcgattaag
aaaaataatcctcgcaagtacctgcggagtgtaggggatggagaggtggtggaatttgatgttgttgctggggaaaagggggcggaggcggccaatgtga
caggcccaaaaggagcaccagtacaaggcagtcgctatgcggcagatcggcgcagataccgccgcggttactatggaaggaggagaggtcccccaaggga
gggaggagacggtgagataaaagatggggttccagaaatggtccagacccagcaaatcaatcgtcagcctgctggctaccgcccaagatatcgccgaccc
atgaatcaaccaagatctattccacctactggagaaatggaaaacaaggagaatcagaatgagactgcagcccctgaccagcaacaactgaatcgccgtg
gattccgaagaccgtacaactaccggcgtagacctaacccaggaactgctccagcacagatgagtgaaaccccagctgagaagcctgtgccagcaagtga
gaaaagcaatgctgcgtaatttgagagacccgggcgtccatcatgtgcaaaatgagagctgagaaaagacagacagac
3' EST: DT530712 [+]attttgccacaactttatgttcttttacaccaactgatttttttttttttaaggcatccatggattctgctggtcatgtgaaaaagcaagcatgctgctg
tataccaaggatgttacataagccatgatggcaagccttgtctcattgcaacagctaggaggggtggctttggtttctggttcaagcatgcaggtataga
ctgggaaaagcaacgtgtaaacgtccttactgtgacgcgagtcacatagttaacagcagtgtcttgtacattataacctgttggctataatagcattgga
atgagcctttgattacattttgggcgctcaaattaaatttggggatcggacggttctttgatgcaaggtgaatattctgagatggaaaggtcagcatact
tttatatagaccaaagctggtcatatataggtaaataccctattcctccatcaatgattatttctttttttggtatcaaaagatcaatgggaatttccaa
taaataaacaatctctttctaagaacatataaagtttgtatccccttcactagtaagtggataagaggtgtctgaatcccatgaattccaagcatatcag
cagcatgctatcctaacagcaagtaggcaggatgtctccctcctgatcattgggcaattgtagctcactgacgaaggaaagtagtgcgaggcctcgtctg
tctgtcttttctcagctctcattttgcacatgatggacgcccgggtctctcaaattacgcagcattgcttttctcacttgctggcacaggcttctcagct
ggggtttcactcatctgtgctggagcagttcctgggttaggtctacgccggtagttgtacggtct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers:
