XB-CLONE-3166272
Clone Name: IMAGE:7858655
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Gene:
spint2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC123083 [+]
CTCAGCTAATCGGGGGGTGATCGAAAATATTTCTGTGACTGCATTCCCTGAGGCGCATCAGGCTTATTGAGAGCAAGGGTACTGATTCGCGTATACCGGT
CAGGCAGAGGCGGGCCCAATATAAACAACTGGGTTTCTAATCAATCAGCAGACTCAGTTTGACTTGCACAGTCCGCCTACGCGGATCCCTATAGTAATGG
CGGCGTGGCTGGGTGGGCTTCTGCTGCTTATATTCCCTTTGGCACTGGCGCAGCAGCCAGCATGTGACGGATACGAGGTGTTGGATGGGTTCTGTCTGCC
AGAAGTGGGTACACCGGCTCGGCAGGCGATGAATGAGCTTGTCTCCTCAGGGAACGCCAGCTCCGAGCAGGAGTGTTGGGAACTTTGCTGCTCCACTGAG
CACTGCGACTTGGCTGTGATGGAAGAGGGAAGCTGCAAACTGATCGGCTGTCTGTATAGTGGCTTCAATATGTGTGAACTGGAGAGCAAGGAAGGAACCA
GAAGTTATCGCAGAAAGGACATTGGGCCAACACCCACCCTGAAAGATTTCTGTTTACCAGAAGCTGTCACAGGACCATGCAGAGCTGCTTTTGAAAGGTG
GTGGTACAACCCAAATACCCAGACCTGCGAAAACTTTACTTATGGGGGCTGCAAAGGGAACCTAAACAATCATATTGGCGAGGAGGTGTGCATGAATAAA
TGTGCTGGAGTAACAGCTGAAATGTCTAATGATATCTTGCCACCAAGTAAACGTATGGCTGAAGCAAATTCAGACCCAGCTTGTACTGGGAAAGGCGTAA
TAGGCAACTGCCGAGCTTCCTTCCCTCGTTGGTACTTTGATGCTGAATCACAGAACTGCATATCTTTTACATATGGTGGATGTGGCGGCACTGAAAACAA
CCATAAATCTGTGCAGGAATGCGCAGACAGGTGTATTGTTTCCAAACCAGAGCCTGCAAAAGTTCAAGCTCCCATGACTGGCAGCTTCAGCGAGTACTGT
GCAGCACCAAGTTTGACTGGTCCGTGCCGTGCTTCTTTCCGTCGCTGGTACTATGATGTAACAACTGCAACCTGTGTTGCATTCACTTACGGAGGATGTC
GTGGGAACAAAAACAACTACCTTTCTGTGGAAGACTGTGTGAAAAATTGTGCTGGACGCTTAGATGATGATCACACTTCAGATCAAACTGTGCTACATCG
TTCCATTACTGCTGTGGTGCTACCTGCATTACTTGCTATCCTGGCTGGAATCTTGCTGCTTGTTATGATTGTGTTCTTTGTCAAGATGGCAAAGAAGAAT
CAGCGGGATGCACATTTTGGAGCCATTTGGAGCCCCATTGATGACAAGGAATGCTTAATGAACAATGCCTACACCCTCTAAACATAGAATTAAATATCCA
CATTAAATACACTCCATGTTGCAACTGTAAAGAAGTGTCATTTTGCAGAGAGGGACATGCAGACAGAACCTACCACATGATTGACAGATTTTAGTTACCT
GACCTTAGGTTTGATATATATTTCTAAAAAAATTAACACCCGCCAATACAAAAAGTGCTATTGAGAGTTAAACTTAAATAGCAATAACATTAATGCATGA
CTCTGCTACTATGTACTGGTTATGGATTCTCAGTTACTAATAGATCTGAGATTTAAACTCATTGAGGCCCAGTAGTTGCTGAAAAAACATTTTAGAACAA
ACAGGCACAACTTATTATTGATCAGCGTGCAGTTTGAGTCAAACATTTTCTGTTCTGAATGCACTAATTTACAGAGCTTCTAATCATCACTATTGCTTAA
ATACTAGTTCCCCAAAAACTCATATTGCAGGTTTTGCACTGAATGCAACAGGAAATTCCCTGGCATGAGCTTAAAAAAACCTCACCATCATGCATCCAGG
CAGCAATAACTAGTGGATTGCTTTTTTCAGCACTTCCCACTGCAGCCAGTTGGGAAGGACTTGTGGCATGTTTTATAGCTTTTTTTTGGATACCTAGGAT
AAATTCTCAGCAGTCTTACAACTTCTGTAATTTTGACATTTAAATTTTCACCTATATAAGTCTGAGTCCCATGAAATAAATTCAACCTTTTTTTTAAGTA
GCTTTTACTTATGCAATTTTGTTGCTACCCTGGCAGCAAGAAAGCCCTTTAAATCTTGCCTAGTAGTGATGAAAACTGCCTTTGTTACAAAAGCAACTTT
TTTTTTTTCCTTATATATATTTTCTGTCCATCAGCACAGACTTAGTTGCTGTTTGCTTTGCTCTCATTTTATTATGTAGAGATGTCAAAATCTTTAAATT
AATTACTGATATTATGTAGTTCTCCTTGTGGGTAGTTACTTTGGTAAGGAAACATTGCCACATCTGGAATGTCACTGTATTTCACTATATTTGTGGAGTC
TTTAAAAGTGTTTATCCCAAATGCTTTTCCTTTTCCTTTTTTTATTAGTCTTGATTTTGGTGAGATTTTGAATCATAAATTGGGTTGTTTGTTTTTAAGG
AGTGACATTTTATTTTTTCATATACAACTTGTAACTTCTAATAAAGGTTAGTCTGGAAAAAAAAAAAAAAAAAA
5' EST: DT403522 [+]ctcagctaatcggggggtgatcgaaaatatttctgtgactgcattccctgaggcgcatcaggcttattgagagcaagggtactgattcgcgtataccggt
caggcagaggcgggcccaatataaacaactgggtttctaatcaatcagcagactcagtttgacttgcacagtccgcctacgcggatccctatagtaatgg
cggcgtggctgggtgggcttctgctgcttatattccctttggcactggcgcagcagccagcatgtgacggatacgaggtgttggatgggttctgtctgcc
agaagtgggtacaccggctcggcaggcgatgaatgagcttgtctcctcagggaacgccagctccgagcaggagtgttgggaactttgctgctccactgag
cactgcgacttggctgtgatggaagagggaagctgcaaactgatcggctgtctgtatagtggcttcaatatgtgtgaactggagagcaaggaaggaacca
gaagttatcgcagaaaggacattgggccaacacccaccctgaaagatttctgtttaccagaagctgtcacaggaccatgcagagctgcttttgaaaggtg
gtggtacaacccaaatacccagacctgcgaaaactttacttatgggggctgcaaagggaacctaaacaatcatattggcgaggaggtgtgcatgaataaa
tgtgctggagtaacagctgaaatgtctaatgatatcttgccaccaagtaaacgtatggctgaagcaaattcagacccagcttgtactgggaaaggcgtaa
taggcaactgccgagcttcccttcctcgntggtactttgatgctgaatcacagaactgcatatcttttacatat
3' EST: DT403521 [+]ccagactaacctttattagaagttacaagttgtatatgaaaaaataaaatgtcactccttaaaaacaaacaacccaatttatgattcaaaatctcaccaa
aatcaagactaataaaaaaaggaaaaggaaaagcatttgggataaacacttttaaagactccacaaatatagtgaaatacagtgacattccagatgtggc
aatgtttccttaccaaagtaactacccacaaggagaactacataatatcagtaattaatttaaagattttgacatctctacataataaaatgagagcaaa
gcaaacagcaactaagtctgtgctgatggacagaaaatatatataaggaaaaaaaaaaagttgcttttgtaacaaaggcagttttcatcactactaggca
agatttaaagggctttcttgctgccagggtagcaacaaaattgcataagtaaaagctacttaaaaaaaaggttgaatttatttcatgggactcagactta
tataggtgaaaatttaaatgtcaaaattacagaagttgtaagactgctgagaatttatcctaggtatccaaaaaaaagctataaaacatgccacaagtcc
ttcccaactggctgcagtgggaagtgctgaaaaaagcaatccactagttattgctgcctggatgcatgatggtgaggtttttttaagctcatgccaggga
atttcctgttgcattcagtgcaaaacctgcaatatgagtttttggggaactagtatttaagcaatagtgatgattagaagctctgtaaattagtgcattc
agaacagaaaatgtttgactcaaactgcacgctgatca
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1745 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: oviduct | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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