XB-CLONE-3181291
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Clone Name: IMAGE:7852728
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Gene:
ybx3
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT393613 
[+]
attgagatccctcgaattaccatctgcatcccaccctccctctccccttcaccctgaaccatctatttcaaatattgggagagaaaaaggggattataca
acatgagtgaagtaacagctgaagaaccaaagcccaaaacaccaggggctgaagcaagttctccatctgcttcaagtgaaatagaacggaaagtgctggc
tactaaagtccagggaactgtgaaatggttcaatgtgagaaacggctatgggttcattaacaggaatgacaccaaagaagatgtctttgtccaccagaca
gcgattaagaaaaataatcctcgcaagtacctgcggagtgtaggggatggagaggtggtggaatttgatgttgttgctggggaaaagggggcggaggcgg
ccaatgtgacaggcccaaaaggagcaccagtacaaggcagtcgctatgcggcagatcggcgcagataccgccgcggttactatggaaggaggagaggtcc
cccaagggagggaggagacggtgagataaaagatggggttccagaaatggtccagacccagcaaatcaatcgtcagcctgctggctaccgcccaagatat
cgccgacccatgaatcaaccaagatctattccacctactggagaaatggaaaacaaggagaatcagaatgagactgcagcccctgaccagcaacaactga
atcgccgtggattccgaagaccgtacaactaccggcgtagacctaacccaggaactgctccagcacaggggagcaaagagtcaaccaagatgagtgaaac
cccagctg
3' EST: DT393612 [+]attttgccacaactttatgttcttttacaccaactgatttttttttttttaaggcatccatggattctgctggtcatgtgaaaaagcaagcatgctgctg
tataccaaggatgttacataagccatgatggcaagccttgtctcattgcaacagctaggaggggtggctttggtttctggttcaagcatgcaggtataga
ctgggaaaagcaacgtgtaaccgtccttactgtgacgcgagtcacatagttaacagcagtgtcttgtacattataacctgttggctataatagcattgga
atgagcctttgattacattttgggcgctcaaattaaatttggggatcggacggttctttgatgcaaggtgaatattctgagatggaaaggtcagcatact
tttatatagaccaaagctggtcatatataggtaaataccctattcctccatcaatgattatttctttttttggtatcaaaagatcaatgggaatttccaa
taaataaacaatctctttctaagaacatataaagtttgtatccccttcactagtaagtggataagaggtgtctgaatcccatgaattccaagcatatcag
cagcatgctatcctaacagcaagtaggcaggatgtctccctcctgatcattgggcaattgtagctcactgacgaaggaaagtagtgcgaggcctcgtctg
tctgtcttttctcagctctcattttgcacatgatggacgcccgggtctctcaaatacgcagcattgcttttctcacttgctggcacaggcttctcagctg
gggtttcactca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers:
