XB-CLONE-3186507
Clone Name: IMAGE:7851297
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Gene:
mcl1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT390166 [+]
catgttttatagctgcacaaatactttttggtaggcagtagaggtggcaaaaaaaaaaccaaacaaattggagatatggtaactgtcataatacaccaac
ttttaaataaatctttcacatagtatttgtacattagctttatcactgctgctttcagatgattttttcattctagtgcatcttcaattgacttaaaatt
gcagtcaataaagaaacccatgtgatttggttgcaccaggaccagaaaaaaaaaaaaaagtgaataaagtaacattagaaacagaaaaatcactaatttg
tgaaagtaaatatctaacagagaatagtaatgactgctccactttcaaaaagggactgaagataatattctgctatgcgtaatgccataaagcagtaagg
tgtacaggctctgatccagggcccgtaatatgaaaccataccaggaaccagcgcaataaatatgcaggaagaaaagtctatttaccaagtagaagcaccc
aaatttctattagacttcatccctttatagataactaccaagcctccacaaaaagaactcaaaatgaaagacatgagctgagcaattccttaatgcatat
taactaaggacatcaaagcagtaaagtgaaattgcatggaagcccgagagctacactctcaagcttcatgggtggtggaacattaacactaaagttccaa
cctgtcaaaagtgctgccatacacctagcaatacattcttattattggtacgtgtacggcaaccttctgatcctgaccaatcattatgtaggatggaagt
tggtcatttcggtttgaaaatgaggaattgaaga
3' EST: DT390165 [+]catcatggcgcccaccccaaggccctggagaccctgcagcgggtcgggggagacattatagagaagcaccatatggcctttactggcatgctgcaaaggt
tgtctatacatagtagagaggacttgcagaaactttccgaagttcccgctttggtctttaatgatggagttacaaactggggccggattgttaccgtcat
aagctttggtgcgtttgttgcaaagcatctaaagagcatagaccttgaagactgtatcatggctttggcggagcacttcacactattcctaatgacaagc
aaaaaagactggataatccaggaaaagggatgggagggctttgtggacttttttcacatagaagactatgaaagtggactcagaactgttttgatggctt
tctcaagtgttgcggttcttggggctggcttggcgttcatgatccggtgaactttgcaatgtatgaacgccgaaaggattttaaatctgcactataaaat
gttctgcaatctttaacatctcctgaacgtgattgtgggactgaaattggaaatagagaaggccagaaccaaggaggcagtaccaccgttggctgcttgt
gctgtcccaaaatgagtttctgagagagtggaaaagacattgccaagtcccctggattgactgtgtcaaatacttttatttttttcccacaaatataata
tattttgagttatttcttagacactatcatgtacctccatgaaaagaaatcacagggaaaggctttgggctggactatgagtaggaagtgggcttgggga
actgcaagttggtcttatgctctgaccaatagactcatgggctgtacagactgactatacagcactttgagctaatttggtgcagtgtgtatgatgg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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