XB-CLONE-3189619
Clone Name: IMAGE:7850107
|
Gene:
btg1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DT388107 [+]
tatcgcttagcactcttcgagggatctgaagtttaggggggcaaacagacccgatcacacatacacctccaagtcttatctattaacttgcacgccatgc
cagggcgcagcttgtaaaaccaccaaggacgtaaaagtgaaaaatataaaaaaaaaaaaattgtaaaacacgaataaaaatccatcaaacaggaccaagc
ggagcgcaatagacgatccgtgctgttccatcggtgttcgtatcgctgtcggtcgcgctcccatgcatacgcactatccctgggccaacatgaagccaga
gatcatggcggcggtgagtttcatctcaaagttcctccgaaccaaaggcctcatgaacgacctcgacctgcagacgtttaaccagtctttacaggagctc
ctggccgatcactataagcatcattggtttccagaaaagccgtcaaggggttcagcctatcgatgtattcggattaaccacaagatggaccctttaattg
gagaggcagcagatcgtattggactcaacagccagcaaatgtttaagcttctgccaagtgaacttactttgtgggttgacccatatgaagtatcgtatcg
cataggagaggatggctctatttgtgtattgtatgaatctgttccaggaggtggtattagtccaagcagcagtggctctctagttgagagtaggatcagc
tgtaaagatgaactcctcttgggccgaacaagcccatccaaaacgtacaacatgatgactgtatctggttaagatatggttgatggctggatcatcttgt
agcagatggaaaccaattattgtttttaatttgggagggctcctatggggatggattgtgaagtttatacaatga
3' EST: DT388106 [+]tttttttttttaaatctatttgcgtaaataacaggctataaaattgccaaagacactgtctaaacaaaggcactttcacttcagaaagatctaccactat
ttgtgtccaatcttcacagctgcgtcattgtataaacttcacaatccatccccataggagccctcccaaattaaaaacaataattggtttccatctgcta
caagatgatccagccatcaaccatatcttaaccagatacagtcatcatgttgtacgttttggatgggcttgttcggcccaagaggagttcatctttacag
ctgatcctactctcaactagagagccactgctgcttggactaataccacctcctggaacagattcatacaatacacaaatagagccatcctctcctatgc
gatacgatacttcatatgggtcaacccacaaagtaagttcacttggcagaagcttaaacatttgctggctgttgagtccaatacgatctgctgcctctcc
aattaaagggtccatcttgtggttaatccgaatacatcgataggctgaaccccttgacggcttttctggaaaccaatgatgcttatagtgatcggccagg
agctcctgtaaagactggttaaacgtctgcaggtcgaggtcgttcatgaggcctttggttcggaggaactttgagatgaaactcaccgccgccatgatct
ctggcttcatgttggcccagggatagtgcgtatgcatgggagcgcgaccgacagcgatacgaacaccgatggaacagcacggatcgtctattgcgctccg
cttggtcctgtttgatggatttttattcgtgttttacaatttt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: