Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Summary Attributions
XB-CLONE-3192198

Clone Name: IMAGE:7886939 Gene: gapdh Species: Xenopus tropicalis  
 

Sequence:

5' EST: DT386437 [+]

3' EST: DT386436 [+]

Library:

Name: NIH_XGC_tropMet8External Dbs: Unigene tropicalis cDNA library, Xenopus IMAGE cDNA Library

Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis (0.5-1.5 kb fraction; NIH_XGC_tropMet7 contains the 1.5-2.5 kb fraction) and ligated into SalI and NotI digested pCMV-SPORT6 vector. Primary library, not amplified. Library constructed at the DOE Joint Genome Institute.

Anatomy: whole organismStage: NF stage 62
Normalized: NoSubtraction: No

Vector DetailsVector Map
Name: pCMV-SPORT6

Type: phagemid

5' Restriction Site: SalI

3' Restriction Site: NotI		Expression Image

Usage:

Suppliers:

Search for clone at: Open Biosystems