XB-CLONE-3246703
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Clone Name: IMAGE:7794417
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Gene:
mamdc2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121969 
[+]
CACCTCAGTGGGGGCAGCGTACTATGTGACATATTTGCCGATGAGGGTGCCAGGTTCCTGTATCCCAGTTACTTGCCTGGAGACAGAGGCAGCTGAGAAC
TCTTTTCTCCTCCCCTTGTGATCTGTCTGTGACCTGCAGCCATCCCTCTCTTCCACCTCCCCCCCACCCAGTCCGCCCCCACGAAGGGGGACAGCTGGGC
TCCATCCCCTATCCATCCAGGAGCGCAGCCCGAAGGCTTTCAGTGCTGCCTGCCCGGTACTCCAAGGGGTAGCTCCTCACAGCGCGGCCATGATGCTCTC
TCACTGGGTACTGCTGCTGTCTCTGGGAGCTGTACAGTTGGCTGAAGGAGCTGAGATTTCTCCTGGATCCTGCACCTTTGAGGACAGCACATGTGCTTAC
ACCTCAGCCTTCCCGTTTCTCCAGTGGACAGTAAACACTGAAGGTCATTATGTTTCTGTGGACTCATCCAATGGATCACGGGGGCAGAAAGCTGTGCTGA
TTAGTCCAGATCTTCATCTTGCAGAATGGAGCTGCCTCAGACTGGTGTACCAAATTGCAGGCTCAGAATCCTCTCCAAGTCCCAGCAGCCTGAATGTGTT
TGTCAGGCCTGAGGGAGAGAGCTTTGATTACCTGTTATGGTCAGCAGAAGAACATTCAGACAGCTGGCTAATATCAAGCATAGATTTAAAGAACACAACA
AAAAGGTTCAAGATCATTCTGGAAGGTGTATTGGGACTGGATGCAATGAGCAGTGTTGCTATCTTTGAAGTGAAAATGTCTACTGGTTATTGCATTGAGT
GTGACTTTGAGGAAAACCATCTTTGCGGGTACATGAACAGTTGGAACCCAAATGTAAACTGGTTTGTTGGAGGAGGCAATGTCAAGAATTCTCATTCTAT
TTTGCCAAGAGACCACACACTAAACAATGAATTTGGACACTACATGTATGTTGATTCCGTTTATGTCAAACATTTTCAAGAAGTTGCCCAGCTGGTCTCT
CCACTAATTACAACACCAATATCAGGCTGCCTTTCTTTTTACTTTCAACTTCAGCGTGAGAGTAGTAATATATTTCTGGTACATACCAGAGATCTGCATG
GTTCCTATGATGAAATCTGGAAAATGGGCCTTGTGAGACAAGGAGAATGGAATCTAGCAGAAGTTGATCTAAATGCGCCTGTCCCATTAGAGGTTATTTT
TGAAGTCGCCTTTAATGGTATCCAAGCAGGCTATGTTGCCTTGGATGATATATCATTTTCCCCAGTATCCTGCAGTGGACAGCAAGGTATGTTCTTTGAT
GCAAGAGAAGCTGGATGTGATTTTGAGGAAAGCATGTGCCATTTTCATCAGGATGATAACAATGGTTCTGGATGGAGCAGAGTCAAAGTAAAACCCAATG
CATACCAAATGGGTGATCACTCAAGTGGACTTGGCTATTTCATGATAGCTAATACAAGGTTTACAGGGCAACCTGGTTACTTTGGACGGCTCTATGGTCC
TTCTTTGCCTGGAAACATTCAGTACTGTCTTCGGTTTTTTTATTCATTATATGGCTTTTACAAAACCACTGACTCCCTGGCTGTTTACATCTTTGAAGAG
AATCATGTAGTCCAGGAAAAAATCTGGTCTGCCCATGAAACACCCAAAGGTGTCTGGCTGCAAGCTGAAATCAGCATACATAAACCAATGCCTTTCAAGG
TTGTATTTGTGAGCTGGTGTAAGAGTTTTTGGGACTGTGGCATTGCAGCAGTTGATGACATATCAGTGAGTATGGGAAGCTGCCAAATATCTGACAGAAT
ACCACCATTGCCTGGAAAATGTACCTTTGAAAAAAATGATTGTGGATTTCAGCAGGAATGGCAAAGGAGAGGTATTTGGCACAGGATACAAGGGAGGACC
CCAACATTCTACACAGGACCAAATGGTGACCACACCTCTGGGGTAGGTTACTACATGTACATCGAGGCCACAAACATGGTGTTTGGACAGAAAGCCAGAC
TAATCTCAAAACAACTTCGGGCTTTCACTGGGAAGCAGTGTTTGACATTTTTTTACCATATGTATGGGGCTGGCACTGGACTGCTAAATGTATATTTGAG
CAAGGAAAGTGATATAAATAAGGATACTCTTCTCTGGACAAGAAAAGGTGAACAGAGTATCACTTGGCTGAAAGCTCAAATAGAATATGAGAGTGAACAG
CAACATAAGATTGTCTTCGAAGCTGTACGTGGCATTTCTATAAGAAGTGACATCGGAATTGATGATATTATGTTTCAGAATGGACCTTGTAGTGATTCTA
GTGACCCTCTACAGTCATCTGGCTATTCAGACAACTTCAATGAAATTGACTTTTAGATCATATGGTTTTTTAGAAGCCATCCAACAGAACAAACAGCATT
ACAGCGTTACAATATTCACAAGTTTACAGTTGTTTTAAACTATAGTTGCTAGCACCACTTTGTTGAGAGTTGTAGTTTACAACAGCTGGAACACCGGGTG
TTTAATAACTGCAGTAGACACTTTAGGAACTGCACATAAATGTTCATATGGAACTCTGACACAGTTTACTGATACTTACCACAAACTCAGAACACTCATG
TTCGTCGACAGAGAATTCTACTCAGGGCTTTTCAAATGTATGTTTGCATGGACTGTCAGTAATACATGTGTGCAGCATTACAGTTGGTCACAAACTCCAC
TGTATAGATAACAATGTATTTTTTTATTCTGATTCTTTTTTTTTTGAAGCACAGATAACAACCCACAAAGATTGTAATAAATGTTTGGAAAATAAAAAAA
AAAAAAAA
5' EST: CX928925 [+]gtgggggcagcgtactatgtgacatatttgccgatgagggtgccaggttcctgtatcccagttacttgcctggagacagaggcagctgagaactcttttc
tcctccccttgtgatctgtctgtgacctgcagccatccctctcttccacctcccccccacccagtccgcccccacgaagggggacagctgggctccatcc
cctatccatccaggagcgcagcccgaaggctttcagtgctgcctgcccggtactccaaggggtagctcctcacagcgcggccatgatgctctctcactgg
gtactgctgctgtctctgggagctgtacagttggctgaaggagctgagatttctcctggatcctgcacctttgaggacagcacatgtgcttacacctcag
ccttcccgtttctccagtggacagtaaacactgaaggtcattatgtttctgtggactcatccaatggatcacgggggcagaaagctgtgctgattagtcc
agatcttcatcttgcagaatggagctgcctcagactggtgtaccaaattgcaggctcagaatcctctccaagtcccagcagcctgaatgtgtttgtcagg
cctgagggagagagctttgattacctgttatggtcagcagaagaacattcagacagctggctaatatcaagcatagatttaaagaacacaacaaaaaggt
tcaagatcattctggaaggtgtattgggactggatgcaatgagcagtgttgctatctttgaagtgaaaatgtctactggttattgcattgagtgtgactt
tgaggaaaaccatctttgcgggtacatgaacagttggaacccanatgtaaactggtttgttgga
3' EST: CX928924 [+]ccaaacatttattacaatctttgtgggttgttatctgtgcttcaaaaaaaaaagaatcagaataaaaaaatacattgttatctatacagtggagtttgtg
accaactgtaatgctgcacacatgtattactgacagtccatgcaaacatacatttgaaaagccctgagtagaattctctgtcgacgaacatgagtgttct
gagtttgtggtaagtatcagtaaactgtgtcagagttccatatgaacatttatgtgcagttcctaaagtgtctactgcagttattaaacacccggtgttc
cagctgttgtaaactacaactctcaacaaagtggtgctagcaactatagtttaaaacaactgtaaacttgtgaatattgtaacgctgtaatgctgtttgt
tctgttggatggcttctaaaaaaccatatgatctaaaagtcaatttcattgaagttgtctgaatagccagatgactgtagagggtcactagaatcactac
aaggtccattctgaaacataatatcatcaattccgatgtcacttcttatagaaatgccacgtacagcttcgaagacaatcttatgttgctgttcactctc
atattctatttgagctttcagccaagtgatactctgttcaccttttcttgtccagagaagagtatccttatttatatcactttccttgctcaaatataca
tttagcagtccagtgccagccccatacatatggtaaaaaaatgtcaaacactgcttcccagtgaaagcccgaagttgttttgagattagtctggctttct
gtccaaacaccatgtttgtggcctcgatgtacatgtagtaacctaccc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: testis | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
