XB-CLONE-3247722
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Clone Name: IMAGE:7695302
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Gene:
cyp7b1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC161410 
[+]
gcagcagcaggagcactacagccccggcagccggaagccgctatgcttgacactctgctttatactgtcaccgggcttgtgctggggggcctcctacttc
tgctcctactaccgagaaggcagcggagagaaggagaaccgcctttggaaaatggttggattccatttattggtttggcttatgagtttcataaaaatgc
cttggaattcctaatctcccgacagcagaaatatggagacatatttacagttcatgttgcagggaaatatataacttttatcatggactcgacccagttc
cagtacgtcattaagcatggcaagcaacttgactttcacgagtttgccaattctttgtcatccagaacttttgaccacccacgcttgacagaagcaaagt
tccctcatctaaatgataaattgcacagaatatacaaaatcatgcagggtagggcattggacaagcttactgacagcatgatgggaaatctccaacgtgt
attcaaatggaaattctcacaagcaacagattggaaagcagaaaaaatgtaccagttctgctgctgtattatgtttgaagccagttttatgacactctat
ggaagagatcctattgcagatggtcacaaagttattagcgaaatcagagaaaaatttacaaagtttgatgccaagttcccctatcttgtcataaacatac
caatcgcgttgctaggagctaccaagaagattcgtgaagaactgatacatttctttttccctaataaaatggaaaaaagatccgaaatctccgaggttgt
ccaggagagaaaaaacgtgctggaacaatatgaactgcaggactatgacagagcagcacatcattttgccttcctttgggcctctgtgggtaacacgatt
ccggcaacattctgggccatgtattaccttgtgaggcacccagaagcacttgcagcagtgcgtgacgaaattgaccatttgctgcagtcaacaggtcaga
agaaggggcctgaatatgacatccatatcaccagagaacagttggacagcatggtcttgctaggtagtgctataaaggaatctttccgtttgtgtgctgc
atccatgaatatccgcttggtccaggaggatttcgatttggagctcgagggtaatcagacaatcagactgagaaaagacgacttcatagccttataccca
ccggcgttacacatggaccccgagatctatgaagatcctgagaggtacaaatacgatcgtttcgtagaaaatggaaaagagaagattcttttctacaaaa
agggcaaaaagctgaaagaataccttatgccattcggctctggcaccagcaagtgtcctggtcggttctttgcaatgaatgagataaagcagttcttggc
tgtattattgatatatgtggatatggagttggttgaacacaaagcacttggccacgataacaggcggtcaggacttggtatactgttgcctaattctgat
attatgtttcgttttaaacctagaacactggatttgtaatttgtttaatcagttgaaattacaaaattcaagtcgaatgaaacattgggtgtaataggtg
tagcattatggccacttagccttgctttggctgatttatgactaaaaaggtgtttggcttcagtagacaatagaaataataataggagcaacatgacatc
gtcccttcctgccatatcatcctatccacacactggtaaatacagttccatgtttctttattgtgtgactaggtggcatttgaactaaataggtcggctt
agcacttatgccattccagctgacacatatttacttggaggaataaatatctacatacttcaagtcatttaaaaatcatatgtacacaaaatactttatt
ttttggtttttatgatttatttttatacatatttagttattgaaaaagcatgaatttgatagagagacagacaaacctttatggcaccagactggcctag
actgggagccaaaataggccctggcatttcaactacatagaggcccaatcagccccccaccagtccaataaatagtgattggcagcccctctggcatttg
ccagaatctatagattaccagtccaatctcaataattaaggataacgagtgcattacacgtagcgattccgatctttcattccaccattagtcgcactaa
agatcattcccaccctcggctgaatcgtcagatatggaggttaaaacaatagaaattctacctccacctgccgattcagccctgaacatagattttgctt
aagcgccttcaatggcgcccgatcaaaatcttttaacctgaccgatcgacgagtcgaccgatatctgcagccttctgagcctccttgcatgtcagtcagt
ggggtggttttgtgtgtattgttgcagtgtgtttggacttctcaataaaacaacaaacaaaggtcctaaatgaatctccttatacatcaagtatggaacc
ttaggtcagaggtttccgaatagcagggcaggcaccctgaggacagtcaaaagcactatatggcaggtggtagcctgaaaggatgagattttggtgagtg
ctgttttttttagatttagatactcatacctattgctggatctcttattcaaaaacatttcataaatctgtgactctgtttcacaggtaagaggggccca
cagagaactttgaacacaaatgatagaaaaaaaacactacattgctactttagggttcttcagtaccatctcttatagagtaagctcatcctcccataca
cattctgcaaatgaccttagaaggaatgtaataaaattaattaacgggaaaaaaatatgggcaaatataaacattttataatttaaatgttgtctttatg
aacccaaaacagtattttgtgacttactttttaagctcaccctacctgaaatttttcgaatacagagcagtgtaaatgtattaaggctttatatttatag
tcattagacagcactgtaattagcactgctccatcccagcactgagggtagggcaattagattgtaggcttcattggggaaggggcccacaccatgcatt
ctctatgtaaagcaatgcaaaatgtgttgtgatatataaataaagaataattttattagtaaaaaaaaaaaaaaa
5' EST: CX924305 [+]cagcagcaggagcactacagccccggcagccggaagccgctatgcttgacactctgctttatactgtcaccgggcttgtgctggggggcctcctacttct
gctcctactaccgagaaggcagcggagagaaggagaaccgcctttggaaaatggttggattccatttattggtttggcttatgagtttcataaaaatgcc
ttggaattcctaatctcccgacagcagaaatatggagacatatttacagttcatgttgcagggaaatatataacttttatcatggactcgacccagttcc
agtacgtcattaagcatggcaagcaacttgactttcacgagtttgccaattctttgtcatccagaacttttgaccacccacgcttgacagaagcaaagtt
ccctcatctaaatgataaattgcacagaatatacaaaatcatgcagggtagggcattggacaagcttactgacagcatgatgggaaatctccaacgtgta
ttcaaatggaaattctcacaagcaacagattggaaagcagaaaaaatgtaccagttctgctgctgtattatgtttgaagccagttttatgacactctatg
gaagagatcctattgcagatggtcacaaagttattagcgaaatcagagaaaaatttacaaagtttgatgccaagttcccctatcttgtcataaacatacc
aatcgcgttgctaggagctaccaagaagattcgtgaagaactgatacatttctttttcctaataaaatgg
3' EST: CX924304 [+]tttttttttttttttactaataaaattattctttatttatatatgacaacacattttgcattgctttacatagagaatgcatggtgtgggccccttcccc
aatgaagcctacaatctaattgccctaccctcagtgctgggatggagcagtgctaattacagtgctgtctaatgactataaatataaagccttaatacat
ttacactgctctgtattcgaaaaatttcaggtagggtgagcttaaaaagtaagtcacaaaatactgttttgggttcataaagacaacatttaaattataa
aatgtttatatttgcccatatttttttcccgttaattaattttattacattccttctaaggtcatttgcagaatgtgtatgggaggatgagcttactcta
taagagatggtactgaagaaccctaaagtagcaatgtagtgttttttttctatcatttgtgttcaaagttctctgtgggcccctcttacctgtgaaacag
agtcacagatttatgaaatgtttttgaataagagatccagcaataggtatgagtatctaaatctaaaaaaaacagcactcaccaaaatctcatcctttca
ggctaccacctgccatatagtgcttttgactgtcctcagggtgcctgccctgctattcggaaacctctgacctaaggttccatacttgatgtataaggag
attcatttaggacctttgtttgttgttttattgagaagtccaaacacactgcaacaatacacacaaaac
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: testis | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
