XB-CLONE-3250865
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Clone Name: IMAGE:7692396
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Gene:
espl1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC166369 
[+]
caggaaatctaaagtctttgaagggagctgactttataaaactaacatcttcatttgaggaggtgactctcttgcactcaaagctcaagctgtgtctctc
agaagtaactgccgatcagttgccagatcagcgagttgtttgtgatcgcgttcttcgtgcctgcaaccactgggcaggtgatccgaattgtcatgtcagt
cactggcagagcttagtgcccctggcagctttggtatgccaaggtttctgtgcatcaagccctcaacgtacccccttctaccttgagaaaattctgtttc
acctgctgaagaacatctctgcacgtgtatcctcaagtcatgcctgtagtaatctgtcagatctcttgtatggccttctaactggctgccctttagagca
gcaaagtgcaggagatttcacagctgtgtcaaagagcagttttcagatcttgtggaagtctgcaggggaccaagaacagaagcaaagtcctagctcttgt
aacagagaagtgctgagccttagattgcatgcactgcgctttttaatgttggcagagggatctgtatcacatggggatgcacctcaaactatatcacgcc
atgcaagatgtgctgcgctggcttttgaacccaagggtaaaccatgcagcaaagaagagacatattttttaagtcagacacttgaagatctccttgtgaa
acccctgttaggacgtggtactttgtctggttcttggtcccgatgcttggttgaactgacactagagagatgccgcagactctgcaaaacctgctgcttc
aagcaaggacgacatgttgttgaacaagcacgtggatgtttagaaaagacagagagatgctgccatgctgggttggcacttttaacctttgctgtggaca
ttcatgaatctggcataccgcctagcattggtgaagttctgtctgaagtgattgaaactcttgatgcttcccaatcctgtggtgatggctggatgtgtca
ggcactcagtgaatgtttccagtttgctgtttcttctttagatgggcgtttcaagcattctgggaagctgaaggttgatgacattttgagccttgcaaag
ttccttgaaaagcatcaccagcttctagaaaagcaagtgaccaaggttcctaaggatgtgacacgacaactgaaatcgttaaagcaacagcaattcaaca
gcctgcagcaattcacaaatgctgcctgtgcctttatgcagagctgcaaggacaaggctaaggatggattgctattgctgcttggagcctgcaaagatgc
tgtttctcggatgcacagttctcttcagggtctacctactcaagaaaccacagagttcttgaacataacagcatcctgtgttcataacttggcctactgg
ttctatagccagaagatgcatcaagaagcctctgacctggtatatccccttagtgcatggcttgcacagagtgctccagatggggaccctgacctggcag
ttgaaaggttgcaccgctgctacagactgcatgtagagagctgcagaaaaagtggtcagattatgaaaggccttgaagctgtgggtctttggctgcgagc
tctacgctctcaagtctcccagcatatggcagagccagtggccctgtgggttagacttaaaatggatggtgctaaaagtggagatgaagacgtaaggctg
catactctaaaagatggccttgttgatcagcacgtggacccggagctgatggtatcacttcttgcggaggagcttcaaggatacaaatcagtccatggcg
atacagggcaggaacgatacaatacaatatgtgatctccttgagctctgccatgaggacagcgaccttagaggccatcgtgcaatatttcttctagagct
ggcccaagtcttgtgttaccatgactacactggacagacagactgctctgccctggatgctgttcaggaagctcttcgacttttaaatgcaacttcaaaa
cccacagaacgtttgcaggacattaaagcccaagctctgctctggcagtatatctgtacccttgaagcaaacatgcgggagggtttggaggagcaaaacc
gtaaggcaaagttagagggtcagaaccagtggacaggtgtggattatgagcccaacgacctgaactatgaagataaactacatgatgaccagtctgtcag
ggatgggatctgctttacattggcaggggagacagggccactgaaggggctagatgaagctcttgatctgtggagcagtctcttatccagctcacaagta
caatacttaaacagtgctgaacaaaccattttttcattgcacctgctcggatcattgtacagactgatgggaaaacctctgcaatccatgcagagctttc
agcttgctggacgcctgtgccactctctacaagatcatattaaggaggttggggctctctgccatctcaccaaaattttattctacttggagagtcctga
atatgctcaagttatccttcagaaggcagaggagatcttaaaacgcgctgatcgtactagtgaaaattacactctggctgagctatcctgtcagcttttg
cgtagtcatctctgcagggtcacacgtcaggttaaggaaggggtagaactgcttctgggtcttcttcagcacccttctctgcagaaatcatcaaaggcat
ggtaccttctcaaagtacaagttcttcaagagctttcattgtttctcatgctcccattagaaaacttaacctctgatctatacaggcagttatgggctca
tgggtgtcataatcctgagactgctctgacagaagcccataagctgctaaggagcatcgtcctcctccttcttggaaatgatgttttggcttcccctaaa
ggttcttcagaagtgcgttttgtggataatggagaaaaccttcttcaaaaatggcaagtccttgctgatctcctcagctgcacccatgatctagtgaaga
ctcttggccgtatgggctgtgtttcagaagccaagtcattttgccttgagggactcagagtggccaaaactctgcagagtatgcggcactgcgctgattt
ccttgtgagaaaagcagaactagagactctgcgctcagaaactcagctctgtgaagaagatttgcagtatgtgctgttcctaatggagtcctgtacagat
tttgcagccaaaagtaaacagaaggaggtcaaaataaagctttgcaaagggaaaccatttcataaggtggacattacagaaacacctccaagtcccccta
aagatgattttctgaaggcagtagatcttcattatgtagaaacaagggatttgtcaaaagcccctgaatctccaacatgcaagccttccctggaaaagaa
ccttccacattttttgtctcatacacaggactgcacttgccctctatgctctgatcttgtactgtccttgctttgcacccagtggttggtggctaaggca
gagaataatactcagagcagaggtttgttaaaatcagccctgaaaagatgcaaggcactaacccagcgcttttctggtattgttcaggaaatacttggta
agggagtcaccaaaccagcagttctaggagttgcatctgaacttatggctagagtgtacctggccatggcatcacagagcccctctaagcttccagaaga
gaccctggaggaaggacttagttttgtgtcttcacgccaagcaactcttcctaagcactggagagcgggccttcttctggccaaagctttgtgctctata
tataatctggctgctaagcatggtggctgtgttgcagatatgtttgttcaattatggggctggcagccacatcagaacggcaaaaaagcagcagacctca
atcacaggaacaagacaaccgctagcattacaaaaaaaaaaaaaaa
5' EST: CX918751 [+]cggtcggattccgggattcgtcgaccccgcgtccgcaggaaatctaaagtctttgaagggagctgactttataaaactaacatcttcatttgaggaggtg
actctcttgcactcaaagctcaagctgtgtctctcagaagtaactgccgatcagttgccagatcagcgagttgtttgtgatcgcgttcttcgtgcctgca
accactgggcaggtgatccgaattgtcatgtcagtcactggcagagcttagtgcccctggcagctttggtatgccaaggtttctgtgcatcaagccctca
acgtacccccttctaccttgagaaaattctgtttcacctgctgaagaacatctctgcacgtgtatcctcaagtcatgcctgtagtaatctgtcagatctc
ttgtatggccttctaactggctgccctttagagcagcaaagtgcaggagatttcacagctgtgtcaaagagcagttttcagatcttgtggaagtctgcag
gggaccaagaacagaagcaaagtcctagctcttgtaacagagaagtgctgagccttagattgcatgcactgcgctttttaatgttggcagagggatctgt
atcacatggggatgcacctcaaactatatcacgccatgcaagatgtgctgcgctggcttttgaacccaagggtaaaccatgcagcaaagaagagacatat
tttttaagtcagacacttgaagatctccttgtgaaacccctgttaggacgtggtactttgtctggttcttggtcccgatgcttggttgaactgacactag
agagatgccgcagactctgcaaaacctgctgcttcaagcaggacgacatgttgttgaacagcacgtggatgttt
3' EST: CX918750 [+]tttttttttttttttgtaatgctagcggttgtcttgttcctgtgattgaggtctgctgcttttttgccgttctgatgtggctgccagccccataattgaa
caaacatatctgcaacacagccaccatgcttagcagccagattatatatagagcacaaagctttggccagaagaaggcccgctctccagtgcttaggaag
agttgcttggcgtgaagacacaaaactaagtccttcctccagggtctcttctggaagcttagaggggctctgtgatgccatggccaggtacactctagcc
ataagttcagatgcaactcctagaactgctggtttggtgactcccttaccaagtatttcctgaacaataccagaaaagcgctgggttagtgccttgcatc
ttttcagggctgattttaacaaacctctgctctgagtattattctctgccttagccaccaaccactgggtgcaaagcaaggacagtacaagatcagagca
tagagggcaagtgcagtcctgtgtatgagacaaaaaatgtggaaggttcttttccagggaaggcttgcatgttggagattcaggggcttttgacaaatcc
cttgtttctacataatgaagatctactgccttcagaaaatcatctttagggggacttggaggtgtttctgtaatgtccaccttatgaaatggtttccctt
tgcaaagctttattttgacctccttctgtttacttttggctgcaaaatctgtacaggactccattaggaacagcacatactgcnaatcttcttcacagag
ctgagtttctgagcgc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: testis | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
