XB-CLONE-3253405
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Clone Name: IMAGE:7690064
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Gene:
srebf2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC161639 
[+]
ggcgtcagttttgtcagtgacaggttcgtgaggttcagtaatagggaaccaagaaccggctcctttcgggagcgctagggttgtcacagcccgggatatc
atatggaactaaacatggaaaatatggagacgctgacggaactcggagatgagctcaccctaggggatattgacgaaatgctgcagtttgtcagtaacca
ggtgggtgattttccagacctgtttgaagagcagctgtgccagagttatcaaggaaataatgcaatggagacagccttacccaaagcttataaccaggca
ccgcagcagccatatactacttcaacacctcagcctcaaacgttacctgtaaaagccccctcacaagtaacaccccagagaaccgcacctcttctccagc
ccagacccgtgattcagtcttctcctcagcctcagctgcagcaacagacagtgatgctcaccccaaatttcagcactgccccacagacccgaatcataca
gcagccactcatctatcagaatgctgctacaagttttcaggtgctgcaacctccagttcaaagtctaatgacaacacagcagatgcagcctgtcacaatt
cagcagcaagtacagacagtccaggcccagagagtcctaactcaagcagctaatggtacaatccaaactctgaccccagcaactactgttcagaccgtta
cccctcaggtccagcaagttcctgtgcttgtgcagccacagataatcaaaactgagtctttggtgcttactgcagtgaaggcagatggcagccctgtgat
gacaactatgcagaatccagcaataacaacacttgctactcctatacagactacagctctccagacccttatgggcagcaacgggactattctaaccaca
atgccagtaatgatgggacaggaaaaaatgcccataaaacaagttccaggaagtctaaagcagccagaggtgccgaaggaaggcgagaggagaacaactc
acaacataatagaaaaaaggtatcgatcatccatcaatgacaaaattatcgagctgaaggacctggtcatgggcactgatgccaagatgcacaaatcagg
ggtcctaaagaaggctattgattatataaaatacttgcaacaagtcaaccagaaactgcgtcaggaaaacatggctttaaaactagccaaccagaaaaac
aaatacttaaaaggtattgatcttagcagtctggtggatacaagcattggaatgaagatggatgaattcaatcaaaacattttgatgatgtctcctccag
catcggattctggttcccctgctgtgttctctccatattctgtggattctgaaccagggagccctcttttggatgatgaaaaggttaaagatgaaccaga
ctcacccacaggactgggtatgatggaccgctcccggatgcttctctgcaccatgacatttctttgcttgtccttcaatcccctgacatctttgttacac
tctgaaagtggccagtatactgagagagtgcaacatggaacagggagaaccatgcttggtattgaaacgtcaggtttttatggcagctggtttgattggc
ttatacccacattaatcctttggcttgttaatggagtgattgttctcagcgtcttcatgaaactgcttatccatggagagcctgtgacccgtctacattc
ccgctcatccgtcaaattctggagacaccgcaagcaggcagatttggatcttgctaagggggattttggtgccgcagcaataaacctgcagacatgcttg
tgtgttttgggacgctcattacctgcctcacggtttgatctggcatgcagcctttcttggaacatcattcggtgtagcctgcaaaagatcagccttgtcc
gttggctgctaaaacactcccctgggtactgcaaaaaagcagagtttcaggatgaggcaacaaccagtgcaagggatgctgccctagtataccataaact
gcaccaattgcatctgacagggaagctgccatctaattggaattgttcaggtctcaaccttgctctctgtgctgtaaacctggcggaatgtgctggcaac
aagatctctccaaatttacttgcagaaatccacctaactacagccattcaaatgaaaaccagtttcccaagtagattccgctttcttactgcatatttcc
ttggctgtgcccaaaatgcttcttctgaggaatctcttcctgaccccgtgcgatggcttgcgcatcctttgggaaaatactttttcatcaattctaactg
ggctctgaagagtgtgactaaagactctctttacacctcaacaagaaacccagctgatccagttacacagattcatcgtgctttttgtgagtctctgctg
gaaaaagcaatgtacaccatggcgaaaccagaaaccagtaaagcaccccctgaagaggagagttgtgagttttccagagctcaggaatatttaaaacttc
tcagtgggtttgcagattcagtgggaaacattgcctctcttcccctcagtggatcttcccccatgtcatctgcagatcctatctgtcgctggtggtattc
tgtatcttccatggctattggctggttacaaggggatgattctgtggtgaaatcacactttgcagaagtagaaagaattcctaagctccttgactctgat
aaccctctggtgaaagcagtgatccatatgtgcagagccatgcaagcagctgtacttggcaaatgtgatggcccacagagctcattctatcattgtgaga
aagccagcacattcttgtggaacagtcttaatatgagcagcactggctcatatgcaaatctcaacaaggtggtgcagcttttaatttgcgaccttctgct
ttcattgcgcacttccttatggcagaaacagtcctcctctagtccagcagccggagaatctattcatgcacccacttctgtcctaacaggattccagcgt
gatctcagcagcctgcgccgtttggctctcacctttaaacctgcccactgcaagcttttccttcatgaagccacagtacgattgatggccggtgcaagtc
caacccgtacacatcagcttctgcaacacagcctgcagaaacgcactgcacttgcaaataaacaaggtgacctggactcattaccggggcagcgggagag
agctacagccattctcctggcctgccgacacctaccactttcattcctgtcgtcccccgggcagagagcagtcatgcttgccgaagctgcccgaactctt
gagaaagtgggcgaccgccgttcttaccacgattgtcagcaaatgatggtaaagctgagtggcggcactgctatggcagcctcctgaaaagaacactcgc
caagatctgtccaataaaacatcaacttttttcaggcaggaacaaacaatgttgtctgggttgaacatattcaatattttaagaggttaaaacccagaga
cttcacagctcagtggggttttcaatgatgtcaactattgagtctcaagaagggatctcctccaggattgtagagataaattgaagtggaccatgctttg
ctttgtaatatacattttctgagcatcacaccttgataatatttaagtctcttttttttatagttcacctcattctcagggtacatgtaatatagtgctt
ttttcagccctcgcctctacaggctcatagcaatgagattgctacagttatatatttcccatttcctacattgaaggcattttcaactgtcactgacatt
tcttatgtcaaagctctactcacacatgtcctgtagctgcatacaaatcccttggttaacatgtaccaaagctagcagaaagaactgaagatcaggacat
aaaactcctgtctttctctgccaaagagctttacattaaaaatatccagatctcctggttaactcttacttgtttagctttcgaaccctacatgatgcac
ggatttgccatatttaaatatggcttttttcttccctgtttaccggtctggtgcagttggcttataggctgagcttgttactgttacctgctccttaaat
accttatgggaaaaaaacaacatattccttgagaaaataaaactgggctgtaaaaaaaaaaaaaaaa
5' EST: CX914255 [+]gcgtcagttttgtcagtgacaggttcgtgaggttcagtaatagggaaccaagaaccggctcctttcgggagcgctagggttgtcacagcccgggatatca
tatggaactaaacatggaaaatatggagacgctgacggaactcggagatgagctcaccctaggggatattgacgaaatgctgcagtttgtcagtaaccag
gtgggtgattttccagacctgtttgaagagcagctgtgccagagttatcaaggaaataatgcaatggagacagccttacccaaagcttataaccaggcac
cgcagcagccatatactacttcaacacctcagcctcaaacgttacctgtaaaagccccctcacaagtaacaccccagagaaccgcacctcttctccagcc
cagacccgtgattcagtcttctcctcagcctcagctgcagcaacagacagtgatgctcaccccaaatttcagcactgccccacagacccgaatcatacag
cagccactcatctatcagaatgctgctacaagttttcaggtgctgcaacctccagttcaaagtctaatgacaacacagcagatgcagcctgtcacaattc
agcagcaagtacagacagtccaggcccagagagtcctaactcaagcagctaatggtacaatccaaactctgaccccagcaactactgttcagaccgttac
ccctcaggtccagcaagttcctgtgcttgtgcagccacagataatcaaaactgagtctttggtgcttactgcagtgaaggcagatggcagccctgtgatg
acaactatgcagaatccagnncataacacacttgctactcctatacagactacagctctccagacccttatgggcagcaacgggactattct
3' EST: CX914254 [+]ttttttttttttttttacagcccagttttattttctcaaggaatatgttgtttttttcccataaggtatttaaggagcaggtaacagtaacaagctcagc
ctataagccaactgcaccagaccggtaaacagggaagaaaaaagccatatttaaatatggcaaatccgtgcatcatgtagggttcgaaagctaaacaagt
aagagttaaccaggagatctggatatttttaatgtaaagctctttggcagagaaagacaggagttttatgtcctgatcttcagttctttctgctagcttt
ggtacatgttaaccaagggatttgtatgcagctacaggacatgtgtgagtagagctttgacataagaaatgtcagtgacagttgaaaatgccttcaatgt
aggaaatgggaaatatataactgtagcaatctcattgctatgagcctgtagaggcgagggctgaaaaaagcactatattacatgtaccctgagaatgagg
tgaactataaaaaaaagagacttaaatattatcaaggtgtgatgctcagaaaatgtatattacaaagcaaagcatggtccacttcaatttatctctacaa
tcctggaggagatcccttcttgagactcaatagttgacatcattgaaaaccccactgagctgtgaagtctctgggttttaacctcttaaaatattgaata
tgttcaacccagacaacattgtttgttcctgcctganaaaagttgatgttttattggacagatcttggcgagtgttcttttcaggaggctgccatagcag
tgc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: testis | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
