XB-CLONE-3253919
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Clone Name: IMAGE:7689647
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Gene:
nfatc3
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC168023 
[+]
gagaggtctttctgcaggagccataaaagttgtttagcgcttggatcgtggaaaatcggagacaggacgggaaagtgatacgcgaaaagtcttgaacacg
ttgtggctccatgagtgccgcgcattgcggttgggaagatttggacttcagggccatcttcggtgaggagactttggagaaccgcgagtcagttgatata
gcaccggatgactgtgcctcatttttcatcttcagcagagaccaggctaattcctcagtaagccagcctgtctgcattccgcacaatgaacaccagatcc
acagttcaacccattcttccgcttcctgcttacaagactacagaacttatgatagttccagtgagatccaagattacacatgcagttcttcctttggtcc
aaaatcctttgaatgtcctagcattcagatcacatctatctcccccagctgccaccaagatattggaacatttgaaaatgcagaaaataatagaccagag
agagattatatggacagacctttgcgggaccatctctgcctacatcttgagccttcctttagagagccatccttaagtcccagtcctgggagcagcatat
cttccaggagctggatttctgatgcttcatcttgtgagtctctctcacatatatatgatgatttggactccgagctaaatgaggctgcagcacggtttac
tctgggatctccacttacctctccaagtggatctcctagagagcatccagtggaaaaaacgtggcatgtctgtagtgtcagtccatcatcaagacagtcc
ccatgtcactcccccagaaatagcattactgatgagaactggctcagcccaagaccatcttcaaaaccaccctctagacctacttccccttgtggaaaaa
gacggcactcgagtgctgatattttttattcaggtttagtctcccctcactattctccagcaacttctcctggcccttctcccagaggcagtatcacaga
agattcttggcttaatgcatcaccccatcctgtgcctagtctttctttgtatcagtgctggcaggaaactgccattccccttaaaaccagaaagacctct
gaagaccatagtgccaggttagcaggaaagacagagctaaacacagaggatctagcttgtttgtcaccaacagtggacatgcctacagatgaaagcgcct
gttcacagcacccagttatacaagattcttccactgatcagtttctgtctgttccttcacactttacatggaacaaacataaatcaggacacacgcctgt
ttttaggacatcatccttaccaccattagattgggcactgcctagccggtatgggcagtatgaactgaaagtagacgtgcagccaaagacacatcaccga
gctcactacgagacagagggcagccgtggagctgttaaggcagtcactggtaatcaccctatagtgaagctaactggttatacagaaaagccactcaact
tacagatgtttattggaacagctgatgaccggtatgtgaggcctcatgccttttaccaggtgcatcgcatcaccgggaaaactgtagccactgccagtca
agagattgttataaccaacaccaaagttttggaaatcccactgctaccagaaaacaatatgactgccagcattgattgtgctggtatattgaaactgcga
aattcagacattgaactaaggaagggtgagactgacattggtagaaagaatacacgtgttcgcctggtgttcagagtacacataccccaaaccagtggga
aggtcctgtcgctgcaagcagcttctgtccccattgaatgttcacaacgttctgctcaggagttaccacaagttgaaaaatatagtataaacagctgctc
agtgaatggaggattggagttggtaataacaggatccaatttcatgccagattcaaaggtgatcttccttgagaaaggacaagacggatgcacacattgg
gaagcagaaggaaagactgtatttaaaaaatgtcagatgaataatatagttgtggaaataccagcatatcacaacaagactatagcagcagacgtccaag
tgcagttttacgtgtgtaacggaaaaagaaaaagaagtcagtctcagagattcaactacactccagttttactgaagcaggagaacttagaagatggaga
tctgcaagaagctacagcagtttctcttgctaaggggagaggcatttctgatcttcgtatgatccataccagcctacttttccaagaccaaagatccatt
tgtaaaactcttaaatcttcatctccacctggtgtagaatgccctacacaaccaacatttacttcttctctaaaacatgagattccccaaatatcaaaaa
ttcaagaaccatgcaccagctcttccccatccactgctgatcctgaaaccatggaaaactatccagtctccatctattcatctccgcagaagcagtccat
gccttaccagtgtttcagtccagatataccggtcttaccgtcatcatctcttactgccatgcctcacactggtcaaccatcttctcacacacatgattgc
aggctaaggggtatttccctatctcctaccattactcaccccagacagaactctgcttcatcccatcccactgacatgtctctgggcattaaagctgagc
ttgtggaccatgatataaactttcagactaaagaccttgaggacatcacattggatgatgtggctgagattataagacgtgacatgtctcattgatgcca
gggatatgccaaaagcccttggaacacagcatatctgaatctgatcacgctgtctggatggagagaacttgtgaagataaattcctctctatatttctga
acatttatttatgaaccacagatttgtatgcttctggcttttatgtcccacaaaattcagagaaatggtaaattggaaatcatgatagaacatcaggtgc
tacatgcactaatgtgcgccatttgcaccagcatcagcctaagtttgcctagatttagtaagtcatagcaactaaccaggtgtttgctttgaatcagctg
gccagtaaatcctacctgctaattagtttctatgggttgctagacaagtagcaaacttaagaacatttactacattaccaccttaggggcagctttatca
aagttcgaaattaaagcttagcgcagtaaaattccaccacttttgatttttcctgagatttctatggcctttttttacagaatgtacaatttgatgaata
tactgctacaaatcccataggaataaatagagaatggtagaattttaatgcgataagctctaatttacactttaataaatcttccccttagtgctttagg
cttgttttttgcactttgagtcttgcctcatttataatgatccctaacaggctcttgtgttggatcaatttaaaccagtgttaaatgttctctgactatt
caaaatacagggtgtaactaaaataaataattgtcggaaatcacaaaaaaaaaaaaaaa
5' EST: CX913308 [+]aggtctttctgcaggagccataaaagttgtttagcgcttggatcgtggaaaatcggagacaggacgggaaagtgatacgcgaaaagtcttgaacacgttg
tggctccatgagtgccgcgcattgcggttgggaagatttggacttcagggccatcttcggtgaggagactttggagaaccgcgagtcagttgatatagca
ccggatgactgtgcctcatttttcatcttcagcagagaccaggctaattcctcagtaagccagcctgtctgcattccgcacaatgaacaccagatccaca
gttcaacccattcttccgcttcctgcttacaagactacagaacttatgatagttccagtgagatccaagattacacatgcagttcttcctttggtccaaa
atcctttgaatgtcctagcattcagatcacatctatctcccccagctgccaccaagatattggaacatttgaaaatgcagaaaataatagaccagagaga
gattatatggacagacctttgcgggaccatctctgcctacatcttgagccttcctttagagagccatccttaagtcccagtcctgggagcagcatatctt
ccaggagctggatttctgatgcttcatcttgtgagtctctctcacatatatatgatgatttggactccgagctaaatgaggctgcagcacggtttactct
gggatctccacttacctctccaagtggatctcctagagagcatccagtg
3' EST: CX913307 [+]tttttttttttttttgtgatttccgacaattatttattttagttacaccctgtattttgaatagtcagagaacatttaacactggtttaaattgatccaa
cacaagagcctgttagggatcattataaatgaggcaagactcaaagtgcaaaaaacaagcctaaagcactaaggggaagatttattaaagtgtaaattag
agcttatcgcattaaaattctaccattctctatttattcctatgggatttgtagcagtatattcatcaaattgtacattctgtaaaaaaaggccatagaa
atctcaggaaaaatcaaaagtggtggaattttactgcgctaagctttaatttcgaactttgataaagctgcccctaaggtggtaatgtagtaaatgttct
taagtttgctacttgtctagcaacccatagaaactaattagcaggtaggatttactggccagctgattcaaagcaaacacctggttagttgctatgactt
actaaatctaggcaaacttaggctgatgctggtgcaaatggcgcacattagtgcatgtagcacctgatgttctatcatgatttccaatttaccatttctc
tgaattttgtgggacataaaagccagaagcatacaaatctgtggttcataaataaatgttcagaaatatagagaggaatttatcttcacaagttctctcc
atccagacagcgtgatcagattcagatatgctgtgttccaagggcttttggcatatccctggcatcaatgagacatgtcacgtcttataatctcagccac
atcatccaatgtgatgtc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: testis | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
