XB-CLONE-3254102
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Clone Name: IMAGE:7689214
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Gene:
rgs12
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121412 
[+]
GGGGAAGGGGAGGCAGGAGGAGGCTCCTATTGTCAACTGGGAAGAAAGTTCATTGTTGGGAGGGAGAGAGAGAACTTTTCAAATGCGATTTGCCTTCTGC
TTGGGGCTTGTGGGCCGGTGCTGCCTGAGGAAGGCTTGTTCTGAGCCGGAGTCTATGGCATGCGTGCTGAGGAACATCTGCCTGGATTCAACGCTATGAA
GAAAAGTCATTTTATCTGAGACCCGCAAATTGACACAAATGTACAGGCCAGCCGACCCTATGAAAAGCCGTCTTAAAATGCAGGTGTCATCGAGACTGAG
AAGTGTGGAGGTGGCAAGGGGAAGAACAGGATATGGTTTCACTCTCTCCGGACAGTCCCCATGTGTGATAAGTAGTGTCATCAAGGAAAGTCCAGCTGAG
TTTGTTGGGCTGAAACCAGGAGACCAGATATGTGCCGTCAATGAGATCAACGTTAAGAATGCATCGCATGAAGACGTGGTGAAACTGATAGGGAAATGTT
CAGGCGTTTTGCATATGGTGATTGCTGAGGTAATACGTCACATAGACTCATGTTCCAGTGATGAGGAACTTGCCTTCTATGAAGGGAAAACTTGTCTGAA
GCCAAGACCTGATTCAAAAGCTTTGGGCATCAATAGAGCCAAGAGGGTTGTCGAGGAAATGCAGTCTGGGGGAATATTTAACATGATTTTTGAAAAGCCT
TCATCTAATGCTGCAAAAGGTGATAATCACGCACCAAAACAGAGGTCCAGATCTATGTCTGCTTCTCATTATGAAACGAGACGGGAAAAGGCGAATAAAG
TGGATGTGCTCTCAAAGGAGGAGATATCAAAGGTGCTGAGCAATGACTGTCTTTTCTCGAATGGCACAGACAATATGGAGGATTTTGAGCTTGACGCAAG
TATTCTAAATGTTGGGATGGTCGTTGGCTACCTCGGCTCCATTGAACTCCCTTCTACGAGCTCAAATCTTGAGTATGACAGTTTGCAGGCCATCCGTGGG
TGCATGAGGAGGCTGAGGGCTGAACAGAAAATACATTCACTGGTGCTGATGAAGATAATGCATGACAGCATCCAGTTGTGCACTGATAAGAATGGCGCAA
TAGCTGTTTATCCTGCTGAAAAGCTAGCATTTAGCGCTGTTTGTCCTGATGACAGAAGGTTTTTTGGCCTTGTCACCATGCAAAGCCCTGACGATCAAAG
CTTGGCCGAAGAAGAAGGGATTTTGAGAACATCGTGCCACGTATTTATGGTAGACCCTGAACTTTTCCATCATAAAATACATCAAGGGATATCCCGCAGA
TTTGGATTTGAATGTACCCCAGATCCAGACACCAATGGATGCCTAGAGTTCCCAACCTCGTCTTTGCCTGTGCTGCAGTTTATCTCTGTGCTGTATAGAG
ACATGGGGGAGCTGATTGAAGGAATGAGAGCCAGGGCTTTTCTGGATAATGATGCAGATGCCCATCAGAATAACAGCACCAGCAGTAACAGTGACAGCGG
CATTGGGAATTTCAACCAAGAAGAGAAGCCCAATCGAGTGTTAGTTGTCGATTTAGGAAGTGCACCGAGCAAGCACAACCCACATGCACTACGGGAGGCT
GCTGGAGGAAGAGCACAAAGCCATCAGGCTGCTCTGCACTGGAATGGTTACCCTAATGAAGAAGAAGGACATTTTTCTTTACAAGGAGCTCACGCTGATA
AGCAGCAAAATTTTAAGAAGCATTTAGGCCCATCAGCCCGTATTGAAGTTCCAGTGGTTCCAACAAGAGGCCCAGTACCGCCATCAAAGAAAGCAACTAC
TTTGGATGGAGGGGGTCAGAGGTGGTTGCCTGTGCATGTGATACGGGACTGGCAACACGGAAATATCAGTGATCAGGAGTCTTACGCAGACTCCACAGAT
GGATGGTCTAGTATTAACTGTGGTACCCTTCCTCCTCCAATGAGCAAAATTCCAGCTGACAGATACCGGGTGGAAGGAAATTTTGGGCAGCCTCAGCCTG
TAACTCGCAAAGTTGAAAGTTGGACTAAAAAAGTGTTTGATGTACATAATAAATTCGGAGCTTCACATAACTCTGCCAGAGCCAGAGAGGATAGAAAGAG
CTCAAAGCCTTCCCACTTGGTTCAAGCCCCACAGCGCTCTTCTGGTAGAAGGTCGTTTGGAAGATCTAAAAGGTTCAGCATCACTCGATCCCTGGATGAC
CTTGAGGCTGCCACAGTCTCGGATGGAGAGCTGAATAGCACTGACCTGAAGGGATGCATCAGTGAGAACAGCCTGAGCAGCAATGCCAGCCTTCCAAGTG
TGCAGAGCTGCAGGAGGCTGGGGGAGCGCAGAGTCGCAAGCTGGGCCGTGTCATTTGAACGCCTATTACAAGATCCTGTTGGTGTCCGGTACTTCACGGA
ATTTTTAAAGAAAGAATTTAGCGAAGAAAACATATTGTTCTGGCAGTCGTGTGAATATTTCAGCCGCATTCCCATCCATGACAAGAATGAGCTCTCCTAC
AGAGCCCGGGAAATCTTCAACAAGTTCCTGAGCAGCAAGGCAACAACTCCAGTTAACATTGATAGCCAAGCGCACTTGGCCGACGATGTGCTCAATGCTC
CTCACCCGGATATGTTTAAAGAGCAGCAACTCCAGATATTTAATCTGATGAAGTTTGACAGCTACACGAGGTTTCTGAAGTCCCACCTGTACCAGGAATG
TATGTTGGCAGAAGTTGAGGGTCGGTCACTCCCAGATCCCCAGGAGGTTCCCAGCAGCCCCACGTCGAAGCACAGCATCAGCTCGGAACGATCCAACATC
TCTACCCCGAAAAAGCAGCTGAGTAAGAAAAACCGTTCCGGAAGATCCCTCAACGAAGAGTCCGGAGGTGAAGACGATAACGAAAAAAAAAAAAAAA
5' EST: CX912934 [+]gggaaggggaggcaggaggaggctcctattgtcaactgggaagaaagttcattgttgggagggagagagagaacttttcaaatgcgatttgccttctgct
tggggcttgtgggccggtgctgcctgaggaaggcttgttctgagccggagtctatggcatgcgtgctgaggaacatctgcctggattcaacgctatgaag
aaaagtcattttatctgagacccgcaaattgacacaaatgtacaggccagccgaccctatgaaaagccgtcttaaaatgcaggtgtcatcgagactgaga
agtgtggaggtggcaaggggaagaacaggatatggtttcactctctccggacagtccccatgtgtgataagtagtgtcatcaaggaaagtccagctgagt
ttgttgggctgaaaccaggagaccagatatgtgccgtcaatgagatcaacgttaagaatgcatcgcatgaagacgtggtgaaactgatagggaaatgttc
aggcgttttgcatatggtgattgctgaggtaatacgtcacatagactcatgttccagtgatgaggaacttgccttctatgaagggaaaacttgtctgaag
ccaagacctgattcaaaagctttgggcatcaatagagccaagagggttgtcgaggaaatgcagtctgggggaatatttaacatgatttttgaaaagcctt
catctaatgctgcaaaaggtgataatcacgcaccanaacagaggtccagatctatgtctgcttctcattatgaaacgagacgggaaaaggcgaataaagt
ggatgtgctctcaaaggaggagatatcanaggtgctgagcaatgactgtcttttctcgaatggcacagacaatatggaggattttgagcttgacgcaagt
attctaaat
3' EST: CX912933 [+]atcgtcttcacctccggactcttcgttgagggatcttccggaacggtttttcttactcagctgctttttcggggtagagatgttggatcgttccgagctg
atgctgtgcttcgacgtggggctgctgggaacctcctggggatctgggagtgaccgaccctcaacttctgccaacatacattcctggtacaggtgggact
tcagaaacctcgtgtagctgtcaaacttcatcagattaaatatctggagttgctgctctttaaacatatccgggtgaggagcattgagcacatcgtcggc
caagtgcgcttggctatcaatgttaactggagttgttgccttgctgctcaggaacttgttgaagatttcccgggctctgtaggagagctcattcttgtca
tggatgggaatgcggctgaaatattcacacgactgccagaacaatatgttttcttcgctaaattctttctttaaaaattccgtgaagtaccggacaccaa
caggatcttgtaataggcgttcaaatgacacggcccagcttgcgactctgcgctcccccagcctcctgcagctctgcacacttggaaggctggcattgct
gctcaggctgttctcactgatgcatcccttcaggtcagtgctattcagctctccatccgagactgtggcagcctcaaggtcatccagggatcgagtgatg
ctgaaccttttagatcttccaaacgaccttctaccagaagagcgctgtggggcttgaaccaagtgggaaggctttgagctctttctatcctctctggctc
tggcagagttatgtgaagc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: testis | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
