XB-CLONE-3286368
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Clone Name: IMAGE:7671981
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Gene:
eif4a1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX880208 
[+]
caatggacccgaaggcatggagcccgatggggtcattgagagcaattggaatgaagtcgttgatagttttgatgacatgagcctatcagagtctctgctt
aggggtatttacgcctatggctttgagaaaccctcagccatccagcagcgagctattatgccttgtatcaagggttatgatgtcattgctcaagcacagt
ctggaactgggaaaactgccacctttgccatttccattcttcaacagatagaactggatatgaaagccacacaggccctggtgttggcaccaacccgtga
gctggcccagcagatccagaaagtagttatggccctcggagattacatgggtgcctcttgccacgcctgcattgggggcaccaacgtaagggccgaagta
cagaagctgcagtcagaagcccctcatatagttgtgggcacccccggcagagtgttcgacatgttaaacaggcgttacctgtcccctaaatacataaaga
tgttcgtgttggatgaagccgacgagatgttgagtagaggtttcaaggatcaaatctacgacatattccagaagctgagcagtaatgcccaggtggtttt
gctctcggccaccatgcctgccgacgtgctcgaggtgaccaagaagttcatgcgggaccccatccggatcctggttaaaaaggaggagctgaccctggag
ggtattaggcagttctacatcaacgtggagcgagaggagtggaagctggacacattgtgtgacctgtacgagacgctgaccatcacgcangccgtaatct
tcat
3' EST: CX880207 [+]ttttttttttttttttcgggtttttctgcccctccgacggcggcgggggcgttagatgaggtcggccacgttcatgggcatctcctcgactgtggtgttg
tagaaagtctctatgtctttcagcgtgcgcttgtcatcctctgtcaccatgttaatggcaactcctttcctaccgaaacggccccctcggccaatcctgt
ggatgtagttctccctgttggtcggcagatcatagttaatgaccagtgagacctgctggacatcaatgcctcgggccagcaaatctgtggtaatcagaac
ccgactggatccagatcggaactccctcataatgacgtctctctccttctggtccatgtcgccgtgcagggcggagacagtgaagtccctcgcgtgcatc
ttttccgtcagccaatccaccttccggcgcgtgtttatgaagattacggcctgcgtgatggtcagcgtctcgtacaggtcacacaatgtgtccagcttcc
actcctctcgctccacgttgatgtagaactgcctaataccctccagggtcagctcctcctttttaaccaggatccggatggggtcccgcatgaacttctt
ggtcacctcgagcacgtcggcaggcatggtggccgagagcaaaaccacctgggcattactgctcagcttctggaatatgtcgtagatttgatccttgaaa
cctctactcaacatctcgtcggcttcatccaacacgaacatctttatgtatttaggggacaggtaacgcctgttttacatgtcgaacactctg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn3 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: brain | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
