XB-CLONE-3289738
|
Clone Name: IMAGE:7668405
|
Gene:
dop1b
|
Species: Xenopus tropicalis
|
|
|
|
Sequence:
Full length sequence: BC136149 
[+]
GTGTCAGTGCGGGTAGGAGGCTGCTGGCTGGCAGGGAGAGGGCTGGGCGCCGCTGAGCAGGGACCCGGGCATGCATGAAAGATGGATCCAGAAGAACAGG
AGCTGCTGGGAGACTACAGATACAGAAATTACTCCTCTGTCATTGAGAAAGCCCTGCGGAACTTTGAGTCTTCAAGCGAATGGGCAGATCTGATATCATC
ACTTGGGAAACTCAACAAGGCTTTGCAGAGCAATATAAAGTACAGCCTGCTCCCCAGGCGCCTTATCGTTAGCAAGCGATTAGCACAATGCCTGCACCCA
GCCCTGCCCAGTGGGGTGCACTTAAAGGCCCTGGAGACCTACGAAATCATATTCAAAATTATTGGAACCAAATGTCTGGCCAAGGACCTCTTCCTCTACA
GCTCTGGCCTATTTCCACTGCTGTCCCACGCAGCCATGTCGGTGAGACCTATTCTCCTGGGTTTGTATGAAACCTACTTTATCCCACTGCAGAGGGCCCT
GCTCCCCAGTCTCCAGGCTTTCATCACTGGATTGCTGCCCGGGTTGGAGGAGGGATCTGAGATTTATGAGAGGACAGACCAGCTGCTCCAGCGTCTCGCC
GTTGTAGTGGGACAAGACGTCTTCTATGGCTCACTCTGGGGAAGTGTCCTGGTCAGCAGCTCCATCCGGCTTCCCGCTTCTCTCTTTGTTGTTAGTCACA
TTAATAAAGATTTATCTGCCCAGTCACAAAGCTACATGCTTGGGAAGGATCAGGAGCTAGCCATAAAATCTCTGTGCGCCTCAGTCGTGGATTCCAATGT
CCTGGTGCAGCGAAATACACTGGAAATTCTTCTATTCTTCTTTCCATTTAACACGTGCCTGGTCCCCAGTGAATGCGCCATGCTGCTGCAGCGATCAGAT
ATGGTGCGAATACTCACCGGATCTATCCAGGCTGTGCTAAGAAGGGACATGTCACTGAACAGGAGGTTGTTTGCCTGGCTGCTGGGTTCTGATATCAAGG
GAAGGTGTGTGGATCCAGAATTAAAAGACTCCCTGGGGGATGAAGAGTACTGCAACCATTTCTTTGCAAAATACTCCAAAGATCTTTTGATTGAGGCTTT
AATGCAGATACTGCACCAGAACACTTTGGACTCAGACACAGAGCAGGGGCTGCTGGTTTATCTGAAACCTTTCCGTATCCTTGTTAGCCTTCTAGACAAA
CCTGAGCTAGGCCCCCCATTGGTGGGAGAACTCTTCCTGGAAGTGATGCGAGCCTTCATTGCTTACTGCAAAGATGCGCTGGGGACACAGATGCAACTCA
GCTACAGCCAAAATGGTGCTCAGCTCATAAGTGCCATTAAGGAAAACAAGAATGCCTCTGAGATTGTGAAAACAGTCAACCTACTGATCTCATCTTTAAG
CTCAGACTTTCTGTGGGATTACATGACCATCTGCTTTGAGGACAGCTTCAGGAGAAAAGACCATGATACCCCTGCTGGTAACTATCTGGGCCGAGCTCCC
AGTATTACAGAAGTTTGCAGTTTGCTGGTATTTCTACTGGACGTGATTCCCCTGGAGCTCTATTCAGAAGTACAGACGCAGTACCTACCTCAGATGCTGA
GTTTCATGGCTCAGTCCCTGTCTGACAACATGGATTCTCTGAGCCTTCCGGAACTGACCGTAGCCCTAAAGACGTGCTTTAAAGTTCTCAGCAAAGTGCA
GATGCCGCCAGCATTTCTGGACATGGAAACACCCAATTCAGACATGGATCCAATGAAAGAATCAGAGAGGTTGAATCCAACTACAGAAGTCGAGGGGGAG
ATTCCAGAAGACGAGCCTGTCTTCCACCCACTCAAGTCAGAGGACAGCGGCATTGGCTTGAGTGCCTCATCCCCGGAACTGTCTCAGCACCTACGGATGC
CCAGGGTGTGCCCAGACAAAGGAGATGTGTGGAAGAAGGGAGGGAGCATTCAGATGACCCTGCACTGTTTCCAGGAGGTGGTGGCCAACTTTGCAAGCAA
ACATCTCTTTAGGATCCAGCTTGAAGGTGAAGGGCAAAACCCCCCTGAGAATGGGGTGATGACTGATAATGAAAAAAAAAAAAAAA
5' EST: CX856066 [+]tgtcagtgcgggtaggaggctgctggctggcagggagagggctgggcgccgctgagcagggacccgggcatgcatgaaagatggatccagaagaacagga
gctgctgggagactacagatacagaaattactcctctgtcattgagaaagccctgcggaactttgagtcttcaagcgaatgggcagatctgatatcatca
cttgggaaactcaacaaggctttgcagagcaatataaagtacagcctgctccccaggcgccttatcgttagcaagcgattagcacaatgcctgcacccag
ccctgcccagtggggtgcacttaaaggccctggagacctacgaaatcatattcaaaattattggaaccaaatgtctggccaaggacctcttcctctacag
ctctggcctatttccactgctgtcccacgcagccatgtcggtgagacctattctcctgggtttgtatgaaacctactttatcccactgcagagggccctg
ctccccagtctccaggctttcatcactggattgctgcccgggttggaggagggatctgagatttatgagaggacagaccagctgctccagcgtctcgccg
ttgtagtgggacaagacgtcttctatggctcactctggggaagtgtcctggtcagcagctccatccggcttcccgcttctctctttgttgttagtcacat
taataaagatttatctgcccagtcacaaagctacatgcttgggaaggatcaggagctagccataaaatctctgtgcgcctcagtcgt
3' EST: CX856065 [+]atcagtcatcaccccattctcaggggggttttgcccttcaccttcaagctggatcctaaagagatgtttgcttgcaaagttggccaccacctcctggaaa
cagtgcagggtcatctgaatgctccctcccttcttccacacatctcctttgtctgggcacaccctgggcatccgtaggtgctgagacagttccggggatg
aggcactcaagccaatgccgctgtcctctgacttgagtgggtggaagacaggctcgtcttctggaatctccccctcgacttctgtagttggattcaacct
ctctgattctttcattggatccatgtctgaattgggtgtttccatgtccagaaatgctggcggcatctgcactttgctgagaactttaaagcacgtcttt
agggctacggtcagttccggaaggctcagagaatccatgttgtcagacagggactgagccatgaaactcagcatctgaggtaggtactgcgtctgtactt
ctgaatagagctccaggggaatcacgtccagtagaaataccagcaaactgcaaacttctgtaatactgggagctcggcccagatagttaccagcaggggt
atcatggtcttttctcctgaagctgtcctcaaagcagatggtcatgtaatcccacagaaagtctgagcttaaagatgagatcagtaggttgactgttttc
acaatctcagaggcattcttgttttccttaatggcacttatgagctgagcaccattttggctgtagctgagttgcatctgtgtccccagcgcatctttgc
agtaagcaatgaa
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn3 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: brain | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
