XB-CLONE-3292744
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Clone Name: IMAGE:7665329
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Gene:
anapc7
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC136241 
[+]
GGCTGTGGCAGTAGCTTGGGGTGGAGTTGTGTGGGATACTGAGTGTAGCGCTAATTGTGAAAATGAATGTGATAGATCATGTGCGGGACATGGCAGCGGC
CGGGCTGCACTCCAATGTGCGCTTACTGAGCAGCCTTCTACTGACTATGAGCAACAACAACCCAGAGATGTTTTCTCCATCACAAAAGTACCAGTTGCTT
GTGTACCACGCGGACTCTCTGTTCCATGACAAGGAATACAGAAACGCTGTCAGCAAGTACACAATGGCTCTGCAGCAAAAGAAAGCATTAACCAAGACTT
CCAAAGTTAGACCAGCAACTGGAAATGCAGCTTCCACCCCACAAAATCAGTGTCTTCCATCAGAGATTGAGGTGAAATATAAAATGGCTGACTGTTACAC
CATGCTCAAACAGGACAAGGATGCCATAGCTATTTTAGATGGAATTCCATCTCGCCAGAGAACCCCAAAGATTAACATGATGTTAGCTAATTTGTACAAG
AAAGCTGGACAGGAACGTTCTGCAGTGACCAGTTACAAGGAGGTTCTAAGGCAGTGCCCACTGGCACTTGATGCCATACTAGGTTTGCTGTCCCTTTCTG
TAAAAGGAGCAGAAGTTGCATCTATGACTCTCAATGTCATCCAGAGTATTCCCAACCTAGACTGGCTTTCTGCCTGGATCAAAGCCTATGCATTTGTGCA
CACTGGAGACAACACCAGAGCTATCAATACTATCTGTTCTCTAGAAAAGAAATCTTTGCTAAGGGACAATGTAGATCTTCTGGGGACCTTGGCAGATTTG
TATTTCCGTGTTGGTGACAATAAAAATGCCATTCTTAAGTTTGAGCAAGCCCAGATGCTGGATCCTTATCTCATAAAAGGAATGGATATATATGGGTACT
TGCTGGCCCGTGAAGGTCGACTGGAAGATGTGGAAAATCTTGGCTGCCGCTTGTTCAATATATCTGATCAGCATGCTGAGCCATGGGTGGTTTCTGGGTG
CCACAGCTTTTATACTAAGAGACACTCCAGAGCCCTGTATTTAGGTGCCAAGGCTATCCAGCTGAACAGCAATAGTGTTCAAGCCTTACTATTAAAGGGG
GCAGCATTAAGAAACATGGGAAGGGTGCAGGAAGCAATAATTCATTTCCGAGAAGCAATTCGATTAGCACCTTGTCGGCTTGACTGCTGTGAAGGTCTCA
TTGAATGCTATTTGGCATCCAGCAGTGTGCGGGAAGCCATGGTGATGGCCAACAATGTCTACAAAACCCTTGGAGCCAACGCACAAACTCTAACTCTGCT
GGCAACTGTGTGCCTGGAAGATCCTGTTACCCAAGAGAAAGCAAAAACATTGTTAGACAAAGCGCTCATTCAGAGACCGGATTATATAAAGGCTGTTGTG
AAGAAAGCGGAGTTACTAAGTCGAGAGCAGAAGTATGAAGAGGGAATTGCCCTGCTGCGTAATACCCTTGCAAACCAGAGTGACTGCGTACTACATCGGA
TGCTAGGAGATTTTCTGGTGGCAGTTAATGAGTTTCAGGAAGCTATGGATCAGTACAGCATCGCCCTCAGTTTGGATCCAAATGACCAAAAATCCTTGGA
GGGAATGCAGAAAAAAAAAAAAAAA
5' EST: CX850735 [+]gctgtggcagtagcttggggtggagttgtgtgggatactgagtgtagcgctaattgtgaaaatgaatgtgatagatcatgtgcgggacatggcagcggcc
gggctgcactccaatgtgcgcttactgagcagccttctactgactatgagcaacaacaacccagagatgttttctccatcacaaaagtaccagttgcttg
tgtaccacgcggactctctgttccatgacaaggaatacagaaacgctgtcagcaagtacacaatggctctgcagcaaaagaaagcattaaccaagacttc
caaagttagaccagcaactggaaatgcagcttccaccccacaaaatcagtgtcttccatcagagattgaggtgaaatataaaatggctgactgttacacc
atgctcaaacaggacaaggatgccatagctattttagatggaattccatctcgccagagaaccccaaagattaacatgatgttagctaa
3' EST: CX850734 [+]tttttttttttttttctgcattccctccaaggatttttggtcatttggatccaaactgagggcgatgctgtactgatccatagcttcctgaaactcatta
actgccaccagaaaatctcctagcatccgatgtagtacgcagtcactctggtttgcaagggtattacgcagcagggcaattccctcttcatacttctgct
ctcgacttagtaactccgctttcttcacaacagcctttatataatccggtctctgaatgagcgctttgtctaacaatgtttttgctttctcttgggtaac
aggatcttccaggcacacagttgccagcagagttagagtttgtgcgttggctccaagggttttgtagacattgttggccatcaccatggcttcccgcaca
ctgctggatgccaaatagcattcaatgagaccttcacagcagtcaagccgacaaggtgctaatcgaattgcttctcggaaatgaattattgcttcctgca
cccttcccatgtttcttaatgctgccccctttaatagtaaggcttgaacactattgctgttcagctggatagccttggcacctaaatacagggctctgga
gtgtctcttagtataaaagctgtggcacccagaaaccacccatggctcagcatgctgatcagatatattgaacaagcggcagccaagattttccacatct
tccagtcgaccttcacgggccagcaagtacccatatatatccattccttttatgagataaggatccagcatctgggcttgctcaaact
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn3 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: brain | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
