XB-CLONE-3292820
|
Clone Name: IMAGE:7665353
|
Gene:
adap1
|
Species: Xenopus tropicalis
|
|
|
|
Sequence:
5' EST: CX850580 
[+]
aaaagatagtatacagacagtgaaccgcactcgccagacaagaagtttattagaaaaaagccctatatatatattataatataacttggaaattgcttaa
agctgaaacagtggcattggctggagcagaggatctgggtactggcctgattactaattatggcactaccatctttgtttttctgcatttactaaatacc
caaacagagagaccaatccaggtaagttaaaaactgggtgtgttttggccacacctgctgcataggccccgcccctgactgcagtcctgtttctgagaat
tttcaggtgcaggtgcagtaaactttaatgtaagtgcaaattacagggggtgacccatgagtggggggtgaaatcagggtggtgcccaaaaaatcagtgt
gagttgacatttctgcccagtaaatgaacaaactcctccgtagaagaacaaaatacaggttgaagggttggtagggattggacaatagctttataatgag
aacatgttggagcgggaatggggataagtaacttagtactgtatgaagatagattcattttttttttaatatcctttccctataagacattgctatttgc
aattctctcagtgggtgctgccatgctgcttgcctcggcataggtactctccgtggattactgtttttgcctgcttgtgctcctgtatatcaggattttg
cattggatgtctatggagaacagttcagtctgaggcagtctgacagcagcagctttttatgcttacggtcaatagaaatgttg
3' EST: CX850579 [+]gaagtaataatttattgatcagaataatatttagtacaactattggcgaccaaacaaatgttcacagcatagttatttacaaacacacaccaatctctgt
gcagctgctatacattcagaatcacatgctatgtacaatacaggggagggcacatgtaggtacattactcatagccccatatgtaataaaaggcacaaag
tttgcccaggtgcagtaacccatagtaaccaataatctgttagcttttaaaccatgtggccaggagatgctacgtgctgactggttgctatgggtaacag
gaactgtagcaaactgtgcaccttttattaaataaccaccatagagcggccaaagcacatccttctctcttgtgaccccaccactcaccgaggcccagat
atataaaagtttcctactaaaattatttttgtaggtgtttattttatatattggggtataatagctctaccaacagaacttctctcaacatttctattga
ccgtaagcataaaaagctgctgctgtcagactgcctcagactgaactgttctccatagacatccaatgcaaaatcctgatatacaggagcacaagcaggc
aaaaacagtaatccacggagagtacctatgccgaggcaagcagcatggcagcacccactgagagaattgcaaatagcaatgtcttatagggaaaggatat
taaaaaaaaaatgaatctatcttcatacagtactaagttacttatccccattcccgctcccacatgttctcattataaagctattgtccaaatccctacc
aaccccttcaccctgtattttgttcttctacggaggagtttgttcatttactgggcagaaatgtcaactcacactga
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn3 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: brain | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
