XB-CLONE-3298709
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Clone Name: IMAGE:7661784
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Gene:
cacnb1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC166170 
[+]
gtgatttggggaatatgtggggggagacacaccaatggtccagcagaacaggatggccaggaccccgtccagctccacacaggagatccagatggatgtg
ttagaggggcacagcctacaggccaaatatgccaagaggaaaagtcgttttaagcgttcagatggcagcaccagctctgataccacctccaacagctttg
tgaggcagggttctgcggagtcatacaccagtcgcccgtcagactcagatgtgtctcttgaggaagatcgggaagccttgaggaaggaggcagagcgaca
tgctttggcacagctggaaaaagcaaagacaaaacctgtggcctttgcagttcggacaaatgtgggatatagcccatcccccggggatgatgcccctgtg
cagggaatgtgcatcaactttgatcccaaagacttccttcacatcaaggagaaatataacaatgattggtggattgggcgtgctgtgcgagaaggctgtg
aagttggatttatccctagtcctgttaagttagagcacatgagacttcttcaggaacagaagatgcgacagagccgactaagctccagtaaatccattgg
taattccaacagtttaggtgatgtggttagtgggacccgcagacctacacccccagctacagctaagcagaggcagaagtcaacagagcacatcccccct
tatgatgttgttccatccatgaggccgatcattctagtgggaccatctttaaagggttatgaggtcacagatatgatgcagaaagctttgtttgatttct
tgaagcaccgatttgatgggaggatttccattactcgggtcactgctgatatttctttggccaaacgttcagtcctgaacaacccaagcaaacataatat
tatggagcggtccaacacacgctccagtcttgctgaggtacagagtgagattgagcgcatctttgaactggctcgaaccctgcagctagttgccttggat
gctgacaccatcaaccacccagctcagcttgctaagacctccttggcacccattattgtctacattaagatcacttctccaaaggtattacaccggctga
taaaatccagaggaaagtcccaagctaagcatctgaatgtacagatggtggcagctgataaattggcacagtgccccccggaaatgtttgacatcatcct
ggatgagaaccaattggaggatgcatgtgaacacctggcagagtacctggaagcctactggaaggccacccatccacccagcatcaacccacccaaccca
ctgcttaaccgtacccttgccactgcagccctggcatccagccccgcgcctgtctccaacctccagggttcctacttggtacacggggagctgaggccgg
atggagcagatcagatgggtatgtacgaggggcaacttcggagaggtcagatgggctctcgcatcctttcccgacatgacacgctggagtcggagacaca
gggcagtcgggactctgcctacacagagccaggtgattcctgcatggacatagagactgacccttatgaggatccagagcctaagagccaaggaggagag
accaggaggctccagcgtcaagggtcttgggaggagctgggtccggatcaagagaacctactgcagaggagaaggagggaatctccaaggccgcaaggga
aaggcagggagcagtactggcatgaaggggatggcaggaacagggggcagctggacagctggggtagagatgtatgtattcgttaggcctgggcataagg
gaaccacacaactcaaacacacacttcccttagcattcaactgtattaccctagcctcttctgcctactgtacagttctgtttcactcctcattctcggc
atcttgcattacatttactccagaagcattgccatctgtaacacccccaatgccctactgccaccacccgttcctttttacacctcagggctcttacatg
tagccactggactttcctctggttctctttaaccatcactgccctgtgatctacggacattcctactggtccttgggatacttaacctgcctgctgcgct
ggtggcgccctctggctgctctcaatgtcagcactgccctgtgactctctctctcactctttgcactatcaatgctacccactgtctctttgcaccaaca
ctgcccttttgcaatctaactgcactgacactgccctatgacactctgcactgacttgtattcattccctgccctttgtgcatttcccacttctctggga
tcaactcacagcactaacagtgcccagtgacccagttactgtaccatgtctacagacctactgattgtacgacattgccttctcatcgcccactatcctt
atcctagcccacttcaagcctggactggcaatctgtggggtctggcaaatggggctgctgaaagatgctatagacagtcactatttattgggctgggggg
gggctgtttgtgcctctgggtactgggaatgccaggggggctgtaaggtgccacagacagtcactatttattgggctggggggctgtttgtgcctctggg
tactgggaatgccaggggggctgtaagatgccataggcagtcactatttattgggctgtggggctgtttgggccaatgtgtacttggaatgccagggcct
attttgatttccagtccaggcctgggccactctcgtagtctcacttccccaacacacattgcactatcaacccagcaattgcacaacgctgtcctcttga
gaacccagtgtcttgtagtaccctctttgcactgccccagtctcagctccctgatttagagaggaggaagaagcccttccaagccagtagcaacccccac
gttccaagctcctcctgcaccccttatagaaacgagtaccaggtctccggcagcctgacctgttgggggtttcggagagaccccaccaccccactcaaca
atgtgccataaactgacttgctggagagaagaatgcataccacgaatgtattacacactttgtgccagaagggacagcctctcttagagctgacgaatta
cccacatatctggaaacattgccccccccccccgtgactagccgggcggaaggagttaacagatgcctgatgtatacacgtgcctgcatatgttgatcat
tttttttgcctgcggttcctttcataataatgattcctgcttggtttgtgttcctaatttgtaccgaccaaagggttttggcattgagtattcccagcac
aagctttttccaaagggacgaagattgtcgttttttcattgtgagttacttttttggggttttttttgggtttttttttggacttgtttgtagtagaatc
ttcagaggcccattctccgctcatattggttcaggcccagaacttccgagggatacagtgagatatatacatatataggtgccgaaagtgtattcatccc
ttcctcagatatctcttgatctagtttatgtgaatatatatatttataaacatgaattcaatgtatgcattgtataaacaagcctcttaaatgatttata
catacatttacacgtatttatacagtcctaattaaaacctggtaagatacttaaaaaaaaaaaaaaa
5' EST: CX843544 [+]tgatttggggaatatgtggggggagacacaccaatggtccagcagaacaggatggccaggaccccgtccagctccacacaggagatccagatggatgtgt
tagaggggcacagcctacaggccaaatatgccaagaggaaaagtcgttttaagcgttcagatggcagcaccagctctgataccacctccaacagctttgt
gaggcagggttctgcggagtcatacaccagtcgcccgtcagactcagatgtgtctcttgaggaagatcgggaagccttgaggaaggaggcagagcgacat
gctttggcacagctggaaaaagcaaagacaaaacctgtggcctttgcagttcggacaaatgtgggatatagcccatcccccggggatgatgcccctgtgc
agggaatgtgcatcaactttgatcccaaagacttccttcacatcaaggagaaatataacaatgattggtggattgggcgtgctgtgcgagaaggctgtga
agttggatttatccctagtcctgttaagttagagcacatgagacttcttcaggaacagaagatgcgacagagccgactaagctccagtaaatccattggt
aattccaacagtttaggtgatgtggttagtgggacccgcagacctacacccccagctacagctaagcagaggcagaagtcaacagagcacatcccccctt
atgatgttgttccatccatgaggccgatcattctagtgggaccatctttaaagggttatgaggtcacagatatgatgcagaaagctttgtttgatttctt
gaagcaccgatttgatgggaggatttccattactcgggtcactgctgatatttctttggccaaacgttcagtccctgaacacccnagcaaacataata
3' EST: CX843543 [+]tttttttttttttttaagtatcttaccaggttttaattaggactgtataaatacgtgtaaatgtatgtataaatcatttaagaggcttgtttatacaatg
catacattgaattcatgtttataaatatatatattcacataaactagatcaagagatatctgaggaagggatgaatacactttcggcacctatatatgta
tatatctcactgtatccctcggaagttctgggcctgaaccaatatgagcggagaatgggcctctgaagattctactacaaacaagtccaaaaaaaaaccc
aaaaaaaaccccaaaaaagtaactcacaatgaaaaaacgacaatcttcgtccctttggaaaaagcttgtgctgggaatactcaatgccaaaaccctttgg
tcggtacaaattaggaacacaaaccaagcaggaatcattattatgaaaggaaccgcaggcaaaaaaaatgatcaacatatgcaggcacgtgtatacatca
ggcatctgttaactccttccgcccggctagtcacggggggggggggcaatgtttccaaatatgtgggtaattcgtcagctctaaaaaaggctgtcccttc
tggcacaaagtgtgtaatacattcgtggtatgcattcttctctccagcaagtcagtttatggcacattgttgagtggg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: brain | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
