XB-CLONE-3300558
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Clone Name: IMAGE:7660186
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Gene:
fbxl16
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC136072 
[+]
GCCTTGGATTGGGCTGCTGCTGCTGCCGGCAGGGTGTTACACTGGCTACAAGATGCTGCCTGACCTGCTTTTGTGCTGATGATGGATTTGCTAGAAGGGT
AAATGCCCCCCTTATTTCTACCCCAATAGATGCACCGACGAGCATTTCCCACATCTTCCAGCTGTTGTGTTTCCATCTCCGCCGCCCCGGAGCCTGTCAT
CCTGCCAGCATGCTTCCCATGAAGTGACCACCGGATCCCAGAACGTTAGAACCATGTCTAACACAAGCAACAGAGACACCAAAACCCAATGTCTACCGCG
TAATGGGCTGGTGAAGATTCCTTCTCCCACCAACGGCCTGGGCTCGGCCAGTATTACCAAGGGGACTCCGGCAGTGAAGAACCGCCTGTGCCAGCCCCCT
TCTGTACCCTCCATCCTCAGCCAAGCCTTCCCCAAGCACGCCGAGCATGTTAACCCTTCCACCCTGCCCATCTTGCCCCCACCCCCCACGCACAGTATCC
CCCTCGGACTGAGTCCTGAAGAGGAGAATCTTTCAATGCCTTGTGACCAATCAGCAGACAGAGTACAGAGTCCTGAGATGAACTGGCACCTTGCCCTAGA
TGAGAAGATTCTTACCCGTCTTTTCTGTTACTTCTCGGCCTGTGAGAAGTGCATTTTGGCTCAGGTGTGCAAGACATGGAGGCGAGTTCTGTACCAGTCC
AGGTTTTGGCTAGGCCTGACCCCTGTCTTGCACGCCAAGGAACTGTACAACGTGCTGCCCGCTGGCGACAAAGAGTTTGTGAACCTGCAAGGATTTGCTG
TGCGGGGATTTGAGTCTTTCTGTCTGGTTGGGGTATCGGATCTGGATATCTGTGAGTTTATTGATAACTACCCGCTTTCCAAAAAGGGAGTCAAGTCTGT
GAGCCTTAAGAGGTCGACAATCACGGATGCAGGACTGGAGGTGATGCTGGAGCAGATGCAGGGAGTGGTACGTCTGGAGCTGTCGGGCTGCAATGACTTC
ACTGAGGCTGGCTTGTGGTCGAGCTTACACGGGCGCATTACATCTCTAAGTGTCAGCGACTGCATCAATGTGGCTGATGATGCGGTGGCAGCCATCTCCC
AGCTCCTGCCCAACTTGGGAGAGCTGAATCTCCAAGCCTATCATGTCACGGACACAGCTCTGGCATATTTTACTGCCAAGCAAGGCCGGGCCACACACAC
TCTCCGTCTGCACTCCTGCTGGGAGATTACCAACCATGGGGTTGTTAATGTTGTCCACAGTCTACCCAACCTGACCGTCCTCAGCCTCTCTGGCTGCTCC
AAGGTGACAGACGATGGTGTGGAGTTAGTAGCGGAGAACCTGCGAAGGCTACGGGGTCTGGACCTGTCTTGGTGCCCACGGCTAACAGACACCGCCTTGG
AGTATATCGCTTGTGACCTGCACAAGCTGGAGGAGCTGGTCTTGGACAGGTGTGTGCGGATCACTGACACGGGACTCAGTTACCTGTCCACAATGCCGTC
TCTCCACAGCCTGTACCTACGCTGGTGCTGCCAGGTGCAAGATTTCGGCCTGAAGCATTTGTTGGCAATGAAAAGCTTACGACTCTTATCTCTGGCAGGA
TGTCCCTTGCTTACCACTACAGGCCTCTCTGGCCTGGTCCAACTGCAAGACTTGGAAGAACTGGAACTGACCAACTGTCCTGGGGCGACTCCGGAGCTCT
TCAAGTATTTCTCCCAGCACCTGCCTCGTTGTGTGGTGATTGAGTAAGATACCAGCAACACACTTTAAGCCGTCTCTTCCAAATCATTGACAGCACTGAC
CATCAGATCGGAGACTTTGGCTTGGGAATCGAGACCAAGCCATTGATTACTGACACGCAAAGAGAGCAAGATCCTGGGAGATATTTCACACTCCGGTTTG
TGAAGGCTTCATTCCATGTCCCGAAGGAAGGGTTGTATATTTCCCACGTCTCATTTCTTGTCTTCTCTGTCGTGGACTGTTTTGCAACTCAAAAGTAGAG
CTGGTTCCCATTCAAACCCCCAGCAGTGTCCTCTATAAGGTTAACATGGCATGAGGATTAGAGCAGTGGAGCTTTACTTGCTCTTATGGAAATGGTGGTT
GTCCATTATTTGAGTTTTGGTGCTTTGGAAGATGGTTAGAGAAGATCTACTGGGACCCACATATAATAATTCCTGGATAATTAAATGCCGACACTGTATT
GGCAATATTGATTGAAGTAGGATATTCTGGGTCCACCAATTATCTGAAGAGGTTTCCATGCAAATTGATGTTGGTATGACTGGTCTGTTCTCTTAGAAAG
AGTAACGAGATGAATGGGATGACCACAAGAAGAGCCTATGTGTTGACCCAATGATAATTATCATCTACTCTGAGCCAGAGTCCTACTCCGCCTTCATTCC
ACTTGGTTTGGACATTTATGCATTTGCTGTGGTTCTACAATCGCAAAGCTCATATGGTCTTTAGGTTTAGTCATCGGTGCTGACAGAGCTTTGCAGCTGC
AATCAAATGGTATATTGTTTGGCTTTTGCAGTTGATTAAGGCAAGGGGGTCACAGGCTGTTCATGCTTAGGGTTATCCTAGGCCTTCTATGGGGAGGAAC
CAGCAGCAGTTATTTTCTTAGTTCTGCCCGGTGATCATTTATTCTAAGTGGATCAAGTCTCGAGCCATCAGTTCTATAAAAAGGACAGTACAGGGATATC
GTGGACATGGAGCAAACATTGACCAGAGGAACCATAAGCAAGATATGCCTTTGTGTATATATGTGTTTGCTAAGGTGGACTGGGGGTCTCCCTCACATTG
CTTCATAGCTTGCCCTAGTGCCTGTACATTTTTTGCCCCTGCTTGTCTAAAATTCAAAACAGAAGATGGAAGACAGTATCACCCACACTTTCCGTCCACA
TCCTGGAAGGAATATTTCTTCTTGAGCTGAACCTGCTCCAAGAAAGTTGCCTGAGGAGCACAAATGGTGATAAAATGAAATGTTTTACCCCCTCTGAGAC
ATAATCCATTAGCCCCCCCTAGTGTCTCTACTTGTCCGTTAGTCTGTGATCTTCTCCCTCAATCTCATTCCAGCAGTAGTCTCCCCTCATCCTTACAAGT
TCTTTTTTCCTGTTCTCTGTCCCAAACTCTATTTGCCCCCAGCTGGCCCAAACTCTATTTGCCCCATGATCACATCCTGCTTTGAAGAGCACTGTTTACA
AAACAATCTCGTGCTATTTCTCACATGCTTTAAATATATTAAAATATCTAATTTTTTCCTCCAAAAAAAAAAAAAAA
5' EST: CX840604 [+]ccttggattgggctgctgctgctgccggcagggtgttacactggctacaagatgctgcctgacctgcttttgtgctgatgatggatttgctagaagggta
aatgccccccttatttctaccccaatagatgcaccgacgagcatttcccacatcttccagctgttgtgtttccatctccgccgccccggagcctgtcatc
ctgccagcatgcttcccatgaagtgaccaccggatcccagaacgttagaaccatgtctaacacaagcaacagagacaccaaaacccaatgtctaccgcgt
aatgggctggtgaagattccttctcccaccaacggcctgggctcggccagtattaccaaggggactccggcagtgaagaaccgcctgtgccagccccctt
ctgtaccctccatcctcagccaagccttccccaagcacgccgagcatgttaacccttccaccctgcccatcttgcccccaccccccacgcacagtatccc
cctcggactgagtcctgaagaggagaatctttcaatgccttgtgaccaatcagcagacagagtacagagtcctgagatgaactggcaccttgccctagat
gagaagattcttacccgtcttttctgttacttctcggcctgtgagaagtgcattttggctcaggtgtgcaagacatggaggcgagttctgtaccagtcca
ggttttggctaggcctgacccctgtcttgcacgccaaggaactgtacaacgtgctgcccgctggcgacaaagagtttgtgaacctgcaaggatttgctgt
gcggggatttgagtctttctgtctggttggngtatcggatctggatatctgtgag
3' EST: CX840603 [+]tttttttttttttttggaggaaaaaattagatattttaatatatttaaagcatgtgagaaatagcacgagattgttttgtaaacagtgctcttcaaagca
ggatgtgatcatggggcaaatagagtttgggccagctgggggcaaatagagtttgggacagagaacaggaaaaaagaacttgtaaggatgaggggagact
actgctggaatgagattgagggagaagatcacagactaacggacaagtagagacactagggggggctaatggattatgtctcagagggggtaaaacattt
cattttatcaccatttgtgctcctcaggcaactttcttggagcaggttcagctcaagaagaaatattccttccaggatgtggacggaaagtgtgggtgat
actgtcttccatcttctgttttgaattttagacaagcaggggcaaaaaatgtacaggcactagggcaagctatgaagcaatgtgagggagacccccagtc
caccttagcaaacacatatatacacaaaggcatatcttgcttatggttcctctggtcaatgtttgctccatgtccacgatatccctgtactgtccttttt
atagaactgatggctcgagacttgatccacttagaataaatgatcaccgggcagaactaagaaaataactgctgctggttcctccccatagaaggcctag
gataaccctaagcatgaacagcctgtgacccccttgccttaatcaactgcaaaagccaaacaatataccatttgattgcagctgcaaagctctgtcagca
ccgatgactaaacctaaagaccatatgagctttgcgaatgtagaaccacagcaaatgcataaatgtccaaaccaagtggaaatgaaggcg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: brain | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
