XB-CLONE-3306041
Clone Name: IMAGE:7658732
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Gene:
g0s2
,
lamb3
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC136179 [+]
GAGAAAGGACTGCACACTGTTTCCACCAGCCCAATGACCACTCCGAAAAAGGCTAACACACTGCCCAACACGTAGATTTTCACCATTTTTCTGTTGGGTT
TCTGGCTCAGCATCTCTTTGGCAAATGGGATCAGTTCGTGGATGGTTTCCATATTGGGTCAAAATGCTTGGGATAGCCAACCTACAAGAGAGAAAAGGCT
TATTATTCTCTGTGCCATTTGGAAGGGAGATGTTACAGAAGCAGCATGTTTACACTGAATTAAAGAACCACAGACGTGTGTATCATTAGCTACAAGGACC
CAGATTCCTGTAAGGTGCAGGTGTGCCGCAGCAGACATGTGGGTATATGCTTTGCTTTTAGTTTTACCGACCCTCCTGCATGCACAGAATGACTGTTCCC
GTGGGGCGTGCTACCCTGCAGCTGGAGACCTTCTCATTGGAAGAGGCAATTACTTAAGAGCCTCCTCAACATGTGGCATTGAAAAGCCAGAAACCTACTG
CAGCCCATATGGAGAGATGCAGATGAAATGCTGTCGCTGTGACTCGCGCCAGCCTTTTACAGAGATTAGTCACCGAATCACTAATGTGCTCTCACCCACT
GGCCATCTTCGTTGGTGGCAGTCTTCCAATGGAATTAATGATGTGTCCCTGCAGTTTGATCTGAACAAAAAGTTTCAGCTCAGTGATGTCATTCTGGATT
TCAGGAGCCCTCGGCCAGCGAGTATGTTAATAGAACGATCCTCAGACCATGGGAAAACCTGGCAGGTTTACCAGTATTTGGCTTATGACTGTGCTGCCTC
TTTCCCACACATCAGACGGGGAGTGCCATACGACTTGCAGGATGTGCGATGCCAGGAGCTTCATGGAGACCCCACACAAGGAGGCAAGATACGATTCAGC
CCAATGGAACTGGCTTTCTCTGTTCCTCTATCCCACAGTCAAAGAGTCCAGCACCTTGCAGATTTCACCAACCTGAGGCTGAATTTCACACAACTTGTGC
GTCTCCCTCCCCGGAGGTATCGCGAACCAAGTACATTTTATGCTGTAGATGTGATGCGTGTGCATGGCAGCTGTTTCTGTTATGGCCATGCAGATCGATG
CATGCCAAGTGGTGCCCTGGACAGAGACCACTATGGCGAAATTCAGGTGTATGATAAATGTATTTGCCAGCACAATACTGTTGGGGAAAACTGTGAACGT
TGTGCTGACCTTTTTCATGACTTGCCTTGGAGGCCAGCCGATGACAACTATCCAAATGAGTGCAGAAGATGCAACTGTAACAATCATGCTGATAAGTGCC
ACTTTGACCCGGCTGTGTATGAAGCCAGTGGCAGAGTGAGTGGGGGTGTGTGTGACAATTGCAGAGATGGAACCACAGGAAGGAACTGCGAGAGGTGCCG
GTCAGACTATTACAGGAATCCCAACAAAGACATGAGCCACAGAGATGCCTGTATCTCATGTGACTGTGACCCAGAGGGGAGTATTGATGGCGGGAGCTGT
GACGATCTGACTGGGCGCTGTAACTGTAAAGAAAATGTTGGTGGGGAACGATGTGACCAGTGCAAGCCAGGATACTACCTCCTGAGTGCTTCCAACCCTC
AGGGCTGCACCAAATGTACCTGTAATCCACAGGGGATGGAGAGAGACCAGCCATGTGACAGTGAAACTGGTCAGTGCAAGTGTCTCCCAAATGTCTTAGG
ACAGCGTTGTGACCAGTGTGCTACAAATCATTGGAATTTGGCCAGCGGGCGAGGCTGTGAGAGGTGTGGGTGTCACCCTAGGAACTCTTACAGCCTCCAG
TGTAACCAGGCTACTGGGCAGTGTCTGTGCCGTGAAGGATTTGGGGGGAGGTTCTGTACAGAGTGCCCTGACAGGATGTATGGCAGCCTACAGACAGGAT
GCAGAGCATGTAACTGTGACTTCCAAGGCACACTTGAGCAAGGCTGTGACAAAGCAAATGGCAGGTGTCTGTGCCGGCCAGGAATTACTGGAGTTCGTTG
TGATTCATGCCAGCGTGGGTACTCTGACCCCTTCCCTACATGCAGAGCCTGTCACCAATGTTTCCACTTCTATGACCATGAAATAAATGCTTTGAGGTCT
CATACGCAATCATTGCTTAACATGACCACTGGTCAAGGGCAAGACTCTGACCTGGGGCTGGGACCACGCATGGCAGCAGTTGAAGCTGCACTTCAGCGCG
TTCAGAGGATTTCCACTGGAACCCCCATTCCAGATAATGAGCTGTCCCGTGCAGAAAGCAAGCTTTCCAGGATGAGAGGTGATGCACAGCAACTGAATAC
CAACCTCCCAGACCCTGAGAGCTTTGCACCTCTTTCAAACAATATACATTCACTTCGGGGTCAGTTTAACAGAATAAATTTACTTTACCAGAGCAAAAAG
GAGCAACATAGCAGTTCCATCAGCAATGATGCCTCAGGTATATTCATGGCTGTTAACAGTGCCTACCAGTCATCATTGGAGTCTGCTAAGAGCATTGCTG
CAGCAGAAGGTGTTTTGTCTCAGTCCAAGGACAGCAGGAGAGTGGCACTGGGGCTAGAAGGAAAATCCACAGAATTATTGGCTGACCTTGAGACACTCAA
GGAAGAACTTTCCTACCCAAACCTCACTCCAACCATCAACAGGATCTGTGAAGGTGTGCGAATAGATCCCTGCACCCCTGAAGAGTGTCCTGGACCTGTA
TGTCAAAGAAACAATAACTCTCTGTGTAAGGTGGGGTCTCTTTGTCGTGGGGCTTTCCCTTTATCCAGTAATGCCTTGAGGAACACAGAGAAAACTTCTC
AAGATCTTCAGGACATTAACAACAAGCTGCAGAAAAATGCATTGATGATTCAGGATGCAGAGAAAACTGCCAGTCAGATTCAGGATAATGCCAAGCAACT
GACAGAACAGGTTAGCAGAGCCAGAAGTCGCATGGAGGAGGATATGGGACACTCACGGCAGTTCATACAGCAAGTCAGAGATTTCCTGACAGAGCCTTCA
ACTGACCCAGCTACGATCCAAGAAGTCAGCGAGTATATTTTGTCTCTCAAGCTACCTACAGATGCCTCCTCCATTCTGAGGAAGATGAATGAGATTCGCT
CTATAGCAGGCAAGCTCCCGAATATCGACAGTGTGCTATCCCAGACAAAAGATGATATAGCCAAAGCCAAGAAACTTCAGAGTGAGGCTGAGCTTGCCAG
GAACAAAGCCAAAGATGTGGAAGATGGGGTGGATGGGGTTATCATGAATCTTAGTCAAGCAAAGACAGCACTTCAGGAAGCTGAGGACAAGATCCGCGGT
TCTAGTTCATCTTTACAACAGATCCAGAACCGCATGCATGAGATTGAGGAGGTTTTAAGCCCTGCTGAGAGAGGACTGACAGACATGCATGATCAGATTC
AGCAGTTCACAGCACAAGTGGAAAAAAAAAAAAAAA
5' EST: CX837680 [+]aaaggactgcacactggtttccaccagcccaatgaccactccgaaaaaggctaacacactgcccaacacgtagattttcaccatttttctgttgggtttc
tggctcagcatctctttggcaaatgggatcagttcgtggatggtttccatattgggtcaaaatgcttgggatagccaacctacaagagagaaaaggctta
ttattctctgtgccatttggaagggagatgttacagaagcagcatgtttacactgaattaaagaaccacagacgtgtgtatcattagctacaaggaccca
gattcctgtaaggtgcaggtgtgccgcagcagacatgtgggtatatgctttgcttttagttttaccgaccctcctgcatgcacagaatgactgttcccgt
ggggcgtgctaccctgcagctggagaccttctcattggaagaggcaattacttaagagcctcctcaacatgtggcattgaaaagccagaaacctactgca
gcccatatggagagatgcagatgaaatgctgtcgctgtgactcgcgccagccttttacagagattagtcaccgaatcactaatgtgctctcacccactgg
ccatcttcgttggtggcagtcttccaatggaattaatgatgtgtccctgcagtttgatctgaacaaaaagtttcagctcagtgatgtcattctggatttc
aggagccctcggccagcgagtatgttaatagaacgatcctcagaccatgggaaaacctggcaggtttaccagtatttggcttatgactgtgctgcctctt
tcccacacatc
3' EST: CX837679 [+]tttttttttttttttcacttgtgctgtgaactgctgaatctgatcatgcatgtctgtcagtcctctctcagcagggcttaaaacctcctcaatctcatgc
atgcggttctggatctgttgtaaagatgaactagaaccgcggatcttgtcctcagcttcctgaagtgctgtctttgcttgactaagattcatgataaccc
catccaccccatcttccacatctttggctttgttcctggcaagctcagcctcactctgaagtttcttggctttggctatatcatcttttgtctgggatag
cacactgtcgatattcgggagcttgcctgctatagagcgaatctcattcatcttcctcagaatggaggaggcatctgtaggtagcttgagagacaaaata
tactcgctgacttcttggatcgtagctgggtcagttgaaggctctgtcaggaaatctctgacttgctgtatgaactgccgtgagtgtcccatatcctcct
ccatgcgacttctggctctgctaacctgttctgtcagttgcttggcattatcctgaatctgactggcagttttctctgcatcctgaatcatcaatgcatt
tttctgcagcttgttgttaatgtcctgaagatcttgagaagttttctctgtgttcctcaaggcattactggataaagggaaagccccacgacaaagagac
cccaccttacacagagagttattgtttctttgacatacaggtccaggacactcttcaggggtgcagggatctattcgcacaccttcacagatcctgttga
tgggtggagtgaggtttgggta
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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