XB-CLONE-3312961
|
Clone Name: IMAGE:7652865
|
Gene:
pole3
|
Species: Xenopus tropicalis
|
|
|
|
Sequence:
Full length sequence: BC135964 
[+]
GGAGGAAGAGCGCGTCTGGTTATTGTTCCTGTCAGTGAGGGGCCCTGAGTTGTAGCTGAGCAGAATGGCCGAGAGACCGGAAGATCTGAACTTGCCCAAC
GCTGTGGTCACGCGGATCATTAAGGAAGCTCTCCCAGAAGGGGTTAATATCTCCAAAGAAGCACGGAGCGCAATATCCCGGGCGGCCAGTGTCTTTGTAT
TGTATGCGACATCTTGCGCCAACAACTTTGCCATGAAGGGGAAGCGGAAGACTCTAAACGCGACTGATGTTTTGGCTGCAATGGAAGAGATGGAATTCCA
GCGCTTTCTGACACCCCTGAAAGAATCCTTGGAAGTTTACAGGCAGGATCAGAAAGGCAAAAAAGAGGCCACGGAGCAGAAGAAGAAAGACAAGGAGAAA
AAGGCCGACTCTGAGGATCAGGACAAGAGTCGGGAAGAGGAGAATGAGGATGAAGATGAGAAGATGGAGGAAGATGAAGTTGTGGAAGAGGAGGAAGTGG
AGAACTGAGCGCAGGAGAGCATGATGGGACTGTGTGGCCCCTCGGGGGGACCTTGTTTTGTCGCGACACTGACTTCCTGATCAGTCAGGCTTTATGGTTG
TAATCATTTACTGCATCGCTGCGCCACTCTTACCCTCCAACCAAGAGCTGTGGGCACTTATTCTCCGGGTTGCACCCAATGCCATTCTGTAGGGGCATCC
CCATGGCAACCACGTGGCACAGCGGCAGTGCCCAGCGGGCAGCTTTATACACGGCCTTTGTAGATAACTGGGGAGTGTCATATTAAATGTTTCTTTTGTT
ACACAAAAAAAAAAAAAAA
5' EST: CX820453 [+]gaggaagagcgcgtctggttattgttcctgtcagtgaggggccctgagttgtagctgagcagaatggccgagagaccggaagatctgaacttgcccaacg
ctgtggtcacgcggatcattaaggaagctctcccagaaggggttaatatctccaaagaagcacggagcgcaatatcccgggcggccagtgtctttgtatt
gtatgcgacatcttgcgccaacaactttgccatgaaggggaagcggaagactctaaacgcgactgatgttttggctgcaatggaagagatggaattccag
cgctttctgacacccctgaaagaatccttggaagtttacaggcaggatcagaaaggcaaaaaagaggccacggagcagaagaagaaagacaaggagaaaa
aggccgactctgaggatcaggacaagagtcgggaagaggagaatgaggatgaagatgagaagatggaggaagatgaagttgtggaagaggaggaagtgga
gaactgagcgcaggagagcatgatgggactgtgtggcccctcggggggaccttgttttgtcgcgacactgacttcctgatcagtcaggctttatggttgt
aatcatttactgcatcgctgcgccactcttaccctccaaccaagagctgtgggcacttattctccgggttgcacccaatgccattctgtaggggcatccc
catggcaaccacgtggcacagcggcagtgcccagcgggcagctttatacacggcctttgtagataactggggagtgtcatattaaatgtttcttttgtta
caca
3' EST: CX820452 [+]aacaaaagaaacatttaatatgacactccccagttatctacaaaggccgtgtataaagctgcccgctgggcactgccgctgtgccacgtggttgccatgg
ggatgcccctacagaatggcattgggtgcaacccggagaataagtgcccacagctcttggttggagggtaagagtggcgcagcgatgcagtaaatgatta
caaccataaagcctgactgatcaggaagtcagtgtcgcgacaaaacaaggtccccccgaggggccacacagtcccatcatgctctcctgcgctcagttct
ccacttcctcctcttccacaacttcatcttcctccatcttctcatcttcatcctcattctcctcttcccgactcttgtcctgatcctcagagtcggcctt
tttctccttgtctttcttcttctgctccgtggcctcttttttgcctttctgatcctgcctgtaaacttccaaggattctttcaggggtgtcagaaagcgc
tggaattccatctcttccattgcagccaaaacatcagtcgcgtttagagtcttccgcttccccttcatggcaaagttgttggcgcaagatgtcgcataca
atacaaagacactggccgcccgggatattgcgctccgtgcttctttggagatattaaccccttctgggagagcttccttaatgatccgcgtgaccacagc
gttgggcaagttcagatcttccggtctctcggccattctgctcagctacaactcagggcccctcactgacaggaacaataaccagacgcgctcttcctc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: brain | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
